Sharma J M
Am J Vet Res. 1981 Mar;42(3):483-6.
Cell suspensions of lymphomas induced by Marek's disease (MD) virus were fractionated by sedimentation at unit gravity on a continuous gradient of bovine fetal serum. Cells in various fractions were examined for MD tumor-associated surface antigen (MATSA) by indirect immunofluorescence, using specific antibody, and for viral infectivity by cocultivating fractionated cells with permissive monolayer cells of duck embryo fibroblasts. Most MATSA-bearing cells in the lymphomas sedimented at a sedimentation velocity of greater than 3.0 mm/hour, whereas smaller, slow-sedimentating cells were generally devoid of MATSA expression. Viral infectivity was associated with MATSA-bearing and MATSA-lacking fractions. Within the limits of the experimental procedures used, this observation provided evidence that presence of MD virus genome in lymphocytes doses not result in concurrent expression of detectable MATSA and that MATSA likely represents another stage of interaction between MD virus and certain lymphocytes.
用牛胎血清连续梯度在单位重力下对马立克氏病（MD）病毒诱导的淋巴瘤细胞悬液进行沉降分离。使用特异性抗体通过间接免疫荧光检查各部分细胞的MD肿瘤相关表面抗原（MATSA），并通过将分离的细胞与鸭胚成纤维细胞的允许单层细胞共培养来检测病毒感染性。淋巴瘤中大多数携带MATSA的细胞沉降速度大于3.0毫米/小时，而较小的、沉降缓慢的细胞通常不表达MATSA。病毒感染性与携带MATSA和不携带MATSA的部分有关。在所使用的实验程序范围内，这一观察结果证明淋巴细胞中MD病毒基因组的存在不会导致可检测到的MATSA同时表达，并且MATSA可能代表MD病毒与某些淋巴细胞之间相互作用的另一个阶段。
