Plumb M A, Nicolas R H, Wright C A, Goodwin G H
Nucleic Acids Res. 1985 Jun 11;13(11):4047-65. doi: 10.1093/nar/13.11.4047.
DNA sequence-specific binding proteins eluted from chicken erythrocyte and thymus nuclei, and fractionated as described by Emerson and Felsenfeld (19), have been investigated by filter binding and footprint analyses. The erythrocyte nuclear protein fraction specifically binds to at least two sites within the 5' flanking chromatin hypersensitive site of the chicken beta A-globin gene, and to a site 5' to the human beta-globin gene. The major chicken beta A globin gene binding site [G)18CGGGTGG) and the human beta-globin gene binding site [TA)6(T)8C(T)4) occur at or near sequences which are hypersensitive to S1 nuclease cleavage in supercoiled plasmids. Downstream, the second chicken beta A-globin gene binding site includes the beta-globin gene CACCC consensus sequence. Filter binding studies also show other sequence specific binding activities to human N-ras and human (but not chicken) c-myc gene sequences.
从鸡红细胞和胸腺细胞核中洗脱出来的、并按照艾默生和费尔森菲尔德（19）所述方法分级分离的DNA序列特异性结合蛋白，已通过滤膜结合和足迹分析进行了研究。红细胞核蛋白组分特异性结合于鸡βA-珠蛋白基因5'侧翼染色质超敏位点内的至少两个位点，以及人β-珠蛋白基因5'端的一个位点。主要的鸡βA珠蛋白基因结合位点[G)18CGGGTGG)与人β-珠蛋白基因结合位点[TA)6(T)8C(T)4)出现在超螺旋质粒中对S1核酸酶切割敏感的序列处或其附近。在下游，第二个鸡βA-珠蛋白基因结合位点包括β-珠蛋白基因的CACCC共有序列。滤膜结合研究还显示出对人N-ras和人（而非鸡）c-myc基因序列的其他序列特异性结合活性。
