Dolan M, Sugarman B J, Dodgson J B, Engel J D
Cell. 1981 Jun;24(3):669-77. doi: 10.1016/0092-8674(81)90093-3.
We have isolated the chicken beta-type globin genes from a library of chicken DNA--lambda Charon 4A recombinant bacteriophage. There are four beta-type genes within this segment of the genome; we believe this represents all of the beta-type genes of the chicken. The recombinant lambda C beta G1 contains the embryonic epsilon- and adult beta-globin genes. The hatching beta H- and embryonic rho-globin genes are found in the recombinant lambda C beta G2. Although lambda C beta G1 and lambda C beta G2 do not physically overlap, we present evidence that all four genes are closely linked and transcribed from the same DNA strand. These experiments demonstrate that the chromosomal regions represented by lambda C beta G1 and lambda C beta G2 lie approximately 1.6 kb apart in the chicken genome. A third recombinant lambda C beta G3 extends the genomic locus studied in the vicinity of the beta-type globin genes to approximately 39 kb. The physical order of the chicken beta-type globin genes within this segment of the chromosome is 5' ... rho--beta H--beta--epsilon ... 3'. This arrangement is unique among the vertebrate beta-type globin gene clusters thus far examined, in that embryonic genes are located at the 5' and 3' ends of the cluster while the hatching and adult genes occupy central positions.
我们已从鸡DNA文库——λ噬菌体Charon 4A重组噬菌体中分离出鸡β型珠蛋白基因。在基因组的这一区域内有四个β型基因；我们认为这代表了鸡的所有β型基因。重组λCβG1包含胚胎期的ε珠蛋白基因和成年期的β珠蛋白基因。孵化期的βH珠蛋白基因和胚胎期的ρ珠蛋白基因存在于重组λCβG2中。虽然λCβG1和λCβG2在物理上并不重叠，但我们提供的证据表明，所有四个基因紧密相连，并从同一条DNA链转录。这些实验表明，在鸡基因组中，由λCβG1和λCβG2代表的染色体区域相距约1.6 kb。第三个重组体λCβG3将在β型珠蛋白基因附近研究的基因组位点扩展到约39 kb。在这一段染色体中，鸡β型珠蛋白基因的物理顺序是5'……ρ——βH——β——ε……3'。在迄今为止所研究的脊椎动物β型珠蛋白基因簇中，这种排列方式是独特的，因为胚胎期基因位于基因簇的5'端和3'端，而孵化期和成年期基因占据中心位置。
