Bhatt P N, Johnson E A, Smith A L, Jacoby R O
Arch Virol. 1981;69(3-4):273-86. doi: 10.1007/BF01317342.
The replication of Banzi virus, flavivirus, was compared in vitro and in vivo in tissues of congenic mice genetically resistant (C3H/RV) or genetically susceptible (C3H/He) to lethal infection. Ultrastructural changes in brains of resistant and susceptible adult mice following intracerebral or intraperitoneal inoculation of virus also were compared. Banzi virus replicated equally well in monolayer cultures of infant and adult brain, stimulated and non-stimulated macrophages and embryonic cells from both strains of mice. Similarly, no significant differences were found between strains in virus growth in brain, spleen or thymus of peripherally-inoculated infant mice. In intracerebrally-inoculated adult mice, virus titers in brains of resistant mice were consistently lower than in susceptible mice. Visualization of virus particles was dependent on virus concentration in tissues. The changes in brain tissues of both strains of mice were similar, differing only in the time of onset which was noted two days later in C3H/RV mice than in C3H/He mice. These results indicate that, in the case of Banzi virus, the phenotypic expression of genetically-determined resistance of lethal flavivirus infection cannot be attributed primarily to the ability of host cells to support virus replication.
在体外以及在对致死性感染具有遗传抗性（C3H/RV）或遗传易感性（C3H/He）的同源系小鼠组织内，对班齐病毒（黄病毒属）的复制情况进行了比较。还比较了经脑内或腹腔接种病毒后，抗性和易感成年小鼠脑内的超微结构变化。班齐病毒在幼年和成年脑单层培养物、受刺激和未受刺激的巨噬细胞以及来自两种品系小鼠的胚胎细胞中复制情况相同。同样，在外周接种的幼年小鼠的脑、脾或胸腺中，两种品系小鼠的病毒生长情况未发现显著差异。在经脑内接种的成年小鼠中，抗性小鼠脑内的病毒滴度始终低于易感小鼠。病毒颗粒的可视化取决于组织中的病毒浓度。两种品系小鼠脑组织的变化相似，只是发病时间不同，C3H/RV小鼠比C3H/He小鼠晚两天出现症状。这些结果表明，就班齐病毒而言，遗传决定的对致死性黄病毒感染的抗性表型表达不能主要归因于宿主细胞支持病毒复制的能力。
