Brendler T, Godefroy-Colburn T, Yu S, Thach R E
J Biol Chem. 1981 Nov 25;256(22):11755-61.
Competition of encephalomyocarditis virus, reovirus, and L-cell mRNAs for a message-discriminatory component was studied in vitro. The data were analyzed qualitatively to determine the relative initiation efficiencies among the various mRNAs. The effects of potassium chloride concentration, magnesium acetate concentration, and m7G methylation on mRNA competition in vitro were also studied. These results were correlated with translation rates in vivo for the same mRNAs, to determine if the sites of competition in vitro and in vivo are the same. It was found that under a particular set of magnesium acetate and potassium chloride concentrations, the order of mRNA initiation efficiencies was the same both in vivo and in vitro, suggesting that the same limiting message-discriminatory factor is regulating initiation rates in both cases. This can only be accomplished in a competitive situation when RNA is in molar excess relative to the discriminatory component.
在体外研究了脑心肌炎病毒、呼肠孤病毒和L细胞mRNA对一种信息识别成分的竞争。对数据进行定性分析以确定各种mRNA之间的相对起始效率。还研究了氯化钾浓度、醋酸镁浓度和m7G甲基化对体外mRNA竞争的影响。将这些结果与相同mRNA在体内的翻译速率相关联，以确定体外和体内的竞争位点是否相同。发现在特定的醋酸镁和氯化钾浓度组合下，mRNA起始效率的顺序在体内和体外是相同的，这表明在两种情况下相同的限制信息识别因子在调节起始速率。只有当RNA相对于识别成分处于摩尔过量的竞争情况下才能实现这一点。
