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来自普通变形杆菌的两种新型限制性核酸内切酶。

Two new restriction endonucleases from Proteus vulgaris.

作者信息

Gingeras T R, Greenough L, Schildkraut I, Roberts R J

出版信息

Nucleic Acids Res. 1981 Sep 25;9(18):4525-36. doi: 10.1093/nar/9.18.4525.

DOI:10.1093/nar/9.18.4525
PMID:6272209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327455/
Abstract

Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.

摘要

已从普通变形杆菌ATCC 13315中分离出两种新型序列特异性内切核酸酶。PvuI识别的序列为：5' C G A T↓C G 3' 3' G C↑T A G C 5'，PvuII识别的序列为：5' C A G↓C T G 3' 3' G T C↑G A C 5'，并如箭头（↓）所示进行切割。PvuI是XorII、RshI和XniI的同裂酶。此前尚未描述过具有PvuII特异性的酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/b2c5cfb88949/nar00411-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/5c417a7d23b5/nar00411-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/17cf71eb2d8d/nar00411-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/f1b9001ec1bd/nar00411-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/7e57739579ae/nar00411-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/b2c5cfb88949/nar00411-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/5c417a7d23b5/nar00411-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/17cf71eb2d8d/nar00411-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/f1b9001ec1bd/nar00411-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/7e57739579ae/nar00411-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/327455/b2c5cfb88949/nar00411-0101-a.jpg

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Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
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Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.通过融合具有活性的限制性内切核酸酶和催化失活的归巢内切核酸酶来创建高度特异性的核酸酶。
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Translational independence between overlapping genes for a restriction endonuclease and its transcriptional regulator.重叠基因之间的转录调控与限制酶的翻译独立性。
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