来自普通变形杆菌的两种新型限制性核酸内切酶。
Two new restriction endonucleases from Proteus vulgaris.
作者信息
Gingeras T R, Greenough L, Schildkraut I, Roberts R J
出版信息
Nucleic Acids Res. 1981 Sep 25;9(18):4525-36. doi: 10.1093/nar/9.18.4525.
Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.
已从普通变形杆菌ATCC 13315中分离出两种新型序列特异性内切核酸酶。PvuI识别的序列为：5' C G A T↓C G 3' 3' G C↑T A G C 5'，PvuII识别的序列为：5' C A G↓C T G 3' 3' G T C↑G A C 5'，并如箭头（↓）所示进行切割。PvuI是XorII、RshI和XniI的同裂酶。此前尚未描述过具有PvuII特异性的酶。
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