三种用于检测人类轮状病毒的电子显微镜技术的比较
Comparison of three electron microscopy techniques for the detection of human rotaviruses.
作者信息
Trépanier P, Alain R, Micusan V, McLaughlin B, Berthiaume L
出版信息
Microbiol Immunol. 1981;25(10):1019-24. doi: 10.1111/j.1348-0421.1981.tb00108.x.
Abstract
Rotavirus detection by direct electron microscopy was compared with direct and indirect immune electron microscopy techniques. The latter two approaches permitted the enumeration of 25 and 103 times more rotaviruses respectively, than direct electron microscopy. Also, 70% and 90% of the virus particles were aggregated by direct and indirect immune electron microscopy techniques respectively, thus facilitating their detection.
摘要
将直接电子显微镜检测轮状病毒的方法与直接免疫电子显微镜技术和间接免疫电子显微镜技术进行了比较。后两种方法分别比直接电子显微镜多检测出25倍和103倍的轮状病毒。此外，直接免疫电子显微镜技术和间接免疫电子显微镜技术分别使70%和90%的病毒颗粒发生聚集，从而便于对其进行检测。
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本文引用的文献
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J Virol Methods. 1981 Feb;2(3):169-74. doi: 10.1016/0166-0934(81)90035-5.
2
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J Virol Methods. 1981 Jun;2(6):367-73. doi: 10.1016/0166-0934(81)90060-4.
3
Detection of rotavirus by serological trapping on antibody-coated electron microscope grids.通过在抗体包被的电子显微镜载网上进行血清学捕获来检测轮状病毒。
J Clin Microbiol. 1980 Jul;12(1):101-4. doi: 10.1128/jcm.12.1.101-104.1980.
4
Rapid detection of human viruses in faeces by a simple and routine immune electron microscopy technique.
J Gen Virol. 1981 Jul;55(Pt 1):223-7. doi: 10.1099/0022-1317-55-1-223.
5
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Virology. 1973 Dec;56(2):652-3. doi: 10.1016/0042-6822(73)90068-8.
6
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Lancet. 1973 Dec 29;2(7844):1497. doi: 10.1016/s0140-6736(73)92760-8.
7
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8
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