Houck J C, Chang C M, Kimball C D
Pharmacology. 1981;23(1):14-23. doi: 10.1159/000137523.
Acid extracts of porcine pancreas acetone powders (but not of other tissues) have been shown to contain material which will react firstly in a radioimmunoassay for beta-endorphin, and secondly, compete with 3H-Naloxone and 3H-enkephalinamide in the radio-receptor assay. This material had a molecular weight of approximately 7,000 as judged by both P-10 and G-50 exclusion chromatography, even in the presence of 4 M urea. The G-50 partially purified and desalted fraction demonstrated a dose-dependent analgesia in mice and also influenced mouse behavior in vivo in a manner analogous to that produced by synthetic human beta-endorphin.
猪胰腺丙酮粉的酸提取物（但其他组织的酸提取物不含）已被证明含有一种物质，该物质首先在β-内啡肽的放射免疫分析中发生反应，其次，在放射受体分析中与3H-纳洛酮和3H-脑啡肽酰胺竞争。通过P-10和G-50排阻色谱法判断，即使在存在4M尿素的情况下，这种物质的分子量约为7000。G-50部分纯化和脱盐的组分在小鼠中表现出剂量依赖性镇痛作用，并且在体内也以类似于合成人β-内啡肽产生的方式影响小鼠行为。
