通过利用附近的转座子Tn10插入来确定大肠杆菌K-12中phoM的图谱位置。
Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion.
作者信息
Wanner B L, Bernstein J
出版信息
J Bacteriol. 1982 Apr;150(1):429-32. doi: 10.1128/jb.150.1.429-432.1982.
Abstract
A phoR strain was constructed with transposon Tn10 inserted near the phoM+ locus. This was done without any prior knowledge of the phoM map location. Subsequently, we defined the phoM map position by screening tetracycline-sensitive (Tcs) derivatives for mutants which were both alkaline phosphatase negative (ther phoR phoM double mutant phenotype) and auxotrophic simultaneously. Some of these mutants were Thr-. Bacteriophage P1-mediated transductions were used to confirm that phoM and its nearby Tn10 insertion were closely linked to thr. Unexpectedly, 7 of 10 mutants analyzed also had mutations unlinked to the phoM-thr-Tn10 region. These may represent a new type of Tn10-promoted molecular event which is caused by transposition of a Tn10 end (IS10).
摘要
构建了一个phoR菌株，转座子Tn10插入到phoM⁺位点附近。这一操作是在对phoM图谱位置毫无先验了解的情况下完成的。随后，我们通过筛选对四环素敏感（Tcs）的衍生物，寻找既为碱性磷酸酶阴性（即phoR phoM双突变体表型）又同时为营养缺陷型的突变体，从而确定了phoM的图谱位置。其中一些突变体是苏氨酸缺陷型（Thr⁻）。利用噬菌体P1介导的转导来证实phoM及其附近的Tn10插入与thr紧密连锁。出乎意料的是，所分析的10个突变体中有7个还具有与phoM-thr-Tn10区域不连锁的突变。这些可能代表了一种由Tn10末端（IS10）转座引起的新型Tn10促进的分子事件。
相似文献
1
Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion.通过利用附近的转座子Tn10插入来确定大肠杆菌K-12中phoM的图谱位置。
J Bacteriol. 1982 Apr;150(1):429-32. doi: 10.1128/jb.150.1.429-432.1982.
2
Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli.大肠杆菌碱性磷酸酶正调控基因（phoM）的克隆与特性分析
Mol Gen Genet. 1984;195(3):381-90. doi: 10.1007/BF00341438.
3
Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region.phoM区域对phoR依赖性细菌碱性磷酸酶克隆变异的控制
J Bacteriol. 1987 Feb;169(2):900-3. doi: 10.1128/jb.169.2.900-903.1987.
4
Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase.利用转座子Tn10对大肠杆菌的非选择基因进行定位：一个影响丙酮酸氧化酶的基因的定位
J Bacteriol. 1982 Sep;151(3):1279-89. doi: 10.1128/jb.151.3.1279-1289.1982.
5
Control of bacterial alkaline phosphatase synthesis and variation in an Escherichia coli K-12 phoR mutant by adenyl cyclase, the cyclic AMP receptor protein, and the phoM operon.通过腺苷酸环化酶、环磷酸腺苷受体蛋白和phoM操纵子对大肠杆菌K-12 phoR突变体中细菌碱性磷酸酶合成及变异的控制
J Bacteriol. 1988 Mar;170(3):1092-102. doi: 10.1128/jb.170.3.1092-1102.1988.
6
Molecular cloning of the wild-type phoM operon in Escherichia coli K-12.大肠杆菌K-12中野生型phoM操纵子的分子克隆
J Bacteriol. 1988 Jan;170(1):279-88. doi: 10.1128/jb.170.1.279-288.1988.
7
Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli.碱性磷酸酶表达受影响的突变体：大肠杆菌中磷酸调节子多个正调控因子的证据
Genetics. 1980 Oct;96(2):353-66. doi: 10.1093/genetics/96.2.353.
8
9
Cloning of phoM, a gene involved in regulation of the synthesis of phosphate limitation inducible proteins in Escherichia coli K12.大肠杆菌K12中参与磷酸盐限制诱导蛋白合成调控的phoM基因的克隆。
Mol Gen Genet. 1984;195(1-2):190-4. doi: 10.1007/BF00332745.
10
Identification of the phoM gene product and its regulation in Escherichia coli K-12.大肠杆菌K-12中phoM基因产物的鉴定及其调控
J Bacteriol. 1984 Jul;159(1):19-25. doi: 10.1128/jb.159.1.19-25.1984.
引用本文的文献
1
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
2
Cloning and characterization of the alkaline phosphatase positive regulatory gene (phoM) of Escherichia coli.大肠杆菌碱性磷酸酶正调控基因（phoM）的克隆与特性分析
Mol Gen Genet. 1984;195(3):381-90. doi: 10.1007/BF00341438.
3
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
4
Identification of the phoM gene product and its regulation in Escherichia coli K-12.大肠杆菌K-12中phoM基因产物的鉴定及其调控
J Bacteriol. 1984 Jul;159(1):19-25. doi: 10.1128/jb.159.1.19-25.1984.
5
Identification of the dye gene product, mutational loss of which alters envelope protein composition and also affects sex factor F expression in Escherichia coli K-12.鉴定该染料基因产物，其突变性缺失会改变包膜蛋白组成，并且还会影响大肠杆菌K-12中的性因子F表达。
Mol Gen Genet. 1984;194(1-2):241-7. doi: 10.1007/BF00383523.
6
D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.大肠杆菌K-12中的D-核糖代谢：遗传学、调控与转运
J Bacteriol. 1984 May;158(2):665-73. doi: 10.1128/jb.158.2.665-673.1984.
7
Regulation of ugp, the sn-glycerol-3-phosphate transport system of Escherichia coli K-12 that is part of the pho regulon.ugp的调控，ugp是大肠杆菌K-12的sn-甘油-3-磷酸转运系统，它是pho调节子的一部分。
J Bacteriol. 1985 Jul;163(1):392-4. doi: 10.1128/jb.163.1.392-394.1985.
8
Control of phoR-dependent bacterial alkaline phosphatase clonal variation by the phoM region.phoM区域对phoR依赖性细菌碱性磷酸酶克隆变异的控制
J Bacteriol. 1987 Feb;169(2):900-3. doi: 10.1128/jb.169.2.900-903.1987.
9
Bacterial alkaline phosphatase clonal variation in some Escherichia coli K-12 phoR mutant strains.一些大肠杆菌K-12 phoR突变菌株中细菌碱性磷酸酶的克隆变异。
J Bacteriol. 1986 Dec;168(3):1366-71. doi: 10.1128/jb.168.3.1366-1371.1986.
10
Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon.大肠杆菌phoM区域的核苷酸序列：四个开放阅读框可能构成一个操纵子。
J Bacteriol. 1986 Oct;168(1):294-302. doi: 10.1128/jb.168.1.294-302.1986.
本文引用的文献
1
Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli.碱性磷酸酶表达受影响的突变体：大肠杆菌中磷酸调节子多个正调控因子的证据
Genetics. 1980 Oct;96(2):353-66. doi: 10.1093/genetics/96.2.353.
2
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase.大肠杆菌K-12的外膜蛋白e与碱性磷酸酶共同调控。
J Bacteriol. 1980 Jul;143(1):151-7. doi: 10.1128/jb.143.1.151-157.1980.
4
Co-regulation in Escherichia coli of a novel transport system for sn-glycerol-3-phosphate and outer membrane protein Ic (e, E) with alkaline phosphatase and phosphate-binding protein.大肠杆菌中甘油-3-磷酸新型转运系统与外膜蛋白Ic（e，E）、碱性磷酸酶和磷酸盐结合蛋白的共同调控。
J Bacteriol. 1980 Jul;143(1):142-50. doi: 10.1128/jb.143.1.142-150.1980.
5
recA-dependent genetic switch generated by transposon Tn10.由转座子Tn10产生的recA依赖性遗传开关。
J Mol Biol. 1980 Dec 5;144(2):215-21. doi: 10.1016/0022-2836(80)90033-9.
6
Cloning and restriction mapping of the alkaline phosphatase structural gene (phoA) of Escherichia coli and generation of deletion mutants in vitro.大肠杆菌碱性磷酸酶结构基因（phoA）的克隆与限制酶图谱绘制以及体外缺失突变体的构建
J Bacteriol. 1981 May;146(2):668-75. doi: 10.1128/jb.146.2.668-675.1981.
7
Use of bacteriophage transposon Mu d1 to determine the orientation for three proC-linked phosphate-starvation-inducible (psi) genes in Escherichia coli K-12.利用噬菌体转座子Mu d1确定大肠杆菌K-12中三个与proC相连的磷酸盐饥饿诱导（psi）基因的方向。
J Bacteriol. 1981 Apr;146(1):93-101. doi: 10.1128/jb.146.1.93-101.1981.
8
Positive selection for loss of tetracycline resistance.对四环素抗性丧失的正向选择。
J Bacteriol. 1980 Aug;143(2):926-33. doi: 10.1128/jb.143.2.926-933.1980.
9
Localized mutagenesis of any specific small region of the bacterial chromosome.细菌染色体任何特定小区域的定位诱变。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158-62. doi: 10.1073/pnas.68.12.3158.
10
Amber mutants of the trpR regulatory gene.色氨酸阻遏蛋白调节基因（trpR）的琥珀突变体
J Mol Biol. 1969 Aug 28;44(1):185-93. doi: 10.1016/0022-2836(69)90413-6.
Zotero 插件