一种用于生成轮状病毒野毒株基因组片段诊断指纹图谱的灵敏方法。

A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus.

作者信息

Clarke I N, McCrae M A

出版信息

J Virol Methods. 1981 Dec;3(5):261-9. doi: 10.1016/0166-0934(81)90063-x.

Abstract

The 3'-terminal labelling procedure for analyzing the genome segment profile of field isolates of rotavirus (Clarke and McCrae, 1981, J. Virol. Methods 2, 203) has been further developed to produce a rapid and sensitive method for generating diagnostic fingerprints from individual species of double-stranded RNA. The fingerprints were obtained by a one-dimensional resolution of overlapping terminally labelled oligonucleotides produced by partial nuclease digestion with a base-specific nuclease. This fingerprinting method should be of great value in characterizing the molecular details of genome segment variation and will facilitate detailed molecular epidemiological studies of this important virus group.

摘要

用于分析轮状病毒野毒株基因组片段图谱的3'末端标记程序（Clarke和McCrae，1981年，《病毒学方法杂志》2，203）已得到进一步改进，从而产生一种快速且灵敏的方法，可从双链RNA的各个种类生成诊断指纹图谱。通过用碱基特异性核酸酶进行部分核酸酶消化产生的重叠末端标记寡核苷酸的一维分辨率获得指纹图谱。这种指纹图谱方法在表征基因组片段变异的分子细节方面应具有重要价值，并将促进对这一重要病毒组进行详细的分子流行病学研究。