Clarke I N, McCrae M A
Infect Immun. 1982 May;36(2):492-7. doi: 10.1128/iai.36.2.492-497.1982.
Detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. The individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled RNA with a base-specific nuclease. This analysis showed that whereas mobility variations were always associated with detectable changes in nucleotide sequence, sequence changes at least as great as those found in segments showing electrophoretic mobility variations were also detected in segments showing no mobility variation. Evidence for the occurrence of genome segment reassortment between viruses in the field was obtained from analyzing the species 11 RNAs from these five isolates. The overall conclusion from these results is that great care is required in the interpretation of simple genome profile analysis of different isolates for epidemiological purposes and that classification of these viruses solely on the basis of genome electropherotype could be misleading.
对五株牛轮状病毒分离株进行了详细的结构研究，这些分离株在聚丙烯酰胺凝胶电泳中其基因组片段的迁移模式表现出变异性。通过用碱基特异性核酸酶对末端放射性标记的RNA进行部分消化，对每个分离株的单个基因组片段进行了表征。该分析表明，虽然迁移率变化总是与核苷酸序列中可检测到的变化相关，但在没有迁移率变化的片段中也检测到了至少与显示电泳迁移率变化的片段中发现的变化一样大的序列变化。通过分析这五株分离株的11型RNA，获得了野外病毒之间发生基因组片段重配的证据。这些结果的总体结论是，在为流行病学目的对不同分离株进行简单的基因组图谱分析时需要格外谨慎，仅基于基因组电泳图谱对这些病毒进行分类可能会产生误导。
