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SAL-TOL体内重组质粒pKF439。

SAL-TOL in vivo recombinant plasmid pKF439.

作者信息

Furukawa K, Miyazaki T, Tomizuka N

出版信息

J Bacteriol. 1985 Jun;162(3):1325-8. doi: 10.1128/jb.162.3.1325-1328.1985.

DOI:10.1128/jb.162.3.1325-1328.1985
PMID:2987190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215927/
Abstract

SAL-TOL in vivo recombinant plasmid pKF439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. Analysis of the gene organization of pKF439 revealed that the 57-kilobase TOL fragment, including the 40-kilobase TOL metabolic region, was inserted into the complete SAL replicon at the position of SmaI-C within XhoI-B of SAL. The molecular size of pKF439 was calculated to be 138 kilobases. pKF439 could be transferred to Pseudomonas putida and Pseudomonas aeruginosa at high frequency, and the transconjugants gained the ability to grow with m-xylene, m-toluate, and salicylate as the sole carbon source.

摘要

在含有各种降解性质粒的细菌混合培养菌株中对体内重组质粒pKF439进行了表征。对pKF439基因组织的分析表明，57千碱基的甲苯操纵子片段，包括40千碱基的甲苯操纵子代谢区域，插入到SAL复制子完整序列中SAL的XhoI - B内的SmaI - C位置。计算得出pKF439的分子大小为138千碱基。pKF439能够以高频转移到恶臭假单胞菌和铜绿假单胞菌中，转接合子获得了以间二甲苯、间甲苯酸和水杨酸作为唯一碳源生长的能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ec/215927/4d393db6c187/jbacter00223-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ec/215927/4d393db6c187/jbacter00223-0478-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97ec/215927/4d393db6c187/jbacter00223-0478-a.jpg

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Science. 1981 Dec 4;214(4525):1133-5. doi: 10.1126/science.7302584.
2
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J Bacteriol. 1982 Apr;150(1):188-94. doi: 10.1128/jb.150.1.188-194.1982.
3
TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: enzyme regulation and DNA structure.
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J Bacteriol. 1989 Oct;171(10):5467-72. doi: 10.1128/jb.171.10.5467-5472.1989.
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