Takayama M, Oya A
Biken J. 1981 Sep;24(3):109-18.
A one-point serum dilution method for determination of neutralizing antibody in human sera to varicella-zoster (V-Z) virus instead of the serial serum dilution method was investigated. Focus counting was performed under a microscope on day 5 to 6 after inoculation of V-Z virus into 6-well plastic trays in which human embryonic lung cells were grown. A table was constructed to estimate the ND50 titers by the per cent reduction of the focus count from the control at only one dilution of test sera. The estimated ND50 values agreed well with those determined by the serial serum dilution method. Test sera showed a slight nonspecific reactivity at low serum dilutions, but reliable results could usually be obtained at a serum dilution of 1:8 or more. This method, which saves materials and labor, was applied to the quantification of neutralizing antibody against V-Z virus in human sera with satisfactory accuracy and reproducibility.
研究了一种用于测定人血清中水痘-带状疱疹（V-Z）病毒中和抗体的单点血清稀释法，而不是连续血清稀释法。将V-Z病毒接种到生长有人胚肺细胞的6孔塑料培养板中，在接种后第5至6天于显微镜下进行病灶计数。构建了一个表格，通过仅在一个测试血清稀释度下相对于对照病灶计数的减少百分比来估计ND50滴度。估计的ND50值与通过连续血清稀释法测定的值非常一致。测试血清在低血清稀释度下显示出轻微的非特异性反应，但通常在血清稀释度为1:8或更高时可获得可靠结果。这种节省材料和劳动力的方法应用于人血清中抗V-Z病毒中和抗体的定量，具有令人满意的准确性和可重复性。
