Brown J M, Leon M A, Lightbody J J
J Immunol. 1976 Nov;117(5 Pt.2):1976-80.
A lectin, isolated from wheat germ by affinity chromatography on chitin, was mitogenic for purified human peripheral blood lymphocytes. Peak incorporation of 3H-thymidine was observed after incubation of lymphocyte cultures with wheat germ mitogen for 7 to 10 days. When lymphocytes were separated into two fractions based on their ability to form rosettes with unsensitized sheep erythrocytes, the mitogen induced a negligible proliferative response in either fraction. Mixing experiments demonstrated a strong response in the T lymphocyte fraction which required the collaboration, but not proliferation, of cells present in the nonrosetting fraction. Stimulation was specifically abolished by addition of N-acetyl-D-glucosamine at initiation of culture.
通过几丁质亲和层析从小麦胚芽中分离出的一种凝集素，对纯化的人外周血淋巴细胞具有促有丝分裂作用。用小麦胚芽促有丝分裂原孵育淋巴细胞培养物7至10天后，观察到3H-胸腺嘧啶核苷的掺入峰值。当根据淋巴细胞与未致敏绵羊红细胞形成玫瑰花结的能力将其分为两部分时，促有丝分裂原在这两部分中均诱导出可忽略不计的增殖反应。混合实验表明，T淋巴细胞部分有强烈反应，这需要非玫瑰花结形成部分中存在的细胞协作，但不需要其增殖。在培养开始时加入N-乙酰-D-葡萄糖胺可特异性消除刺激作用。
