新型阿片肽α-新内啡肽化学合成基因在大肠杆菌中的表达
Expression in Escherichia coli of chemically synthesized gene for a novel opiate peptide alpha-neo-endorphin.
作者信息
Tanaka S, Oshima T, Ohsue K, Ono T, Oikawa S, Takano I, Noguchi T, Kangawa K, Minamino N, Matsuo H
出版信息
Nucleic Acids Res. 1982 Mar 11;10(5):1741-54. doi: 10.1093/nar/10.5.1741.
Abstract
Chemically synthesized alpha-neo-endorphin gene was fused to the Escherichia coli beta-galactosidase gene on the plasmid pKO13. The resulting recombinant DNA was used to transform E. coli cells. Radioimmunoassay for alpha-neo-endorphin in CNBr-treated bacterial cells showed that alpha-neo-endorphin was synthesized at approximately 5 x 10(5) molecules per single E. coli cell. One of the transformants, WA802/p alpha NE2, was used for alpha-neo-endorphin purification. From 10.9 g of wet cells, we isolated 4 mg of chemically pure and biologically active alpha-neo-endorphin.
摘要
将化学合成的α-新内啡肽基因与质粒pKO13上的大肠杆菌β-半乳糖苷酶基因融合。所得重组DNA用于转化大肠杆菌细胞。对经溴化氰处理的细菌细胞中的α-新内啡肽进行放射免疫测定表明，每个大肠杆菌细胞约合成5×10⁵个α-新内啡肽分子。其中一个转化体WA802/pαNE2用于α-新内啡肽的纯化。从10.9克湿细胞中，我们分离出4毫克化学纯且具有生物活性的α-新内啡肽。
相似文献
1
Expression in Escherichia coli of chemically synthesized gene for a novel opiate peptide alpha-neo-endorphin.新型阿片肽α-新内啡肽化学合成基因在大肠杆菌中的表达
Nucleic Acids Res. 1982 Mar 11;10(5):1741-54. doi: 10.1093/nar/10.5.1741.
2
Expression of chemically synthesized alpha-neo-endorphin gene fused to E. coli alkaline phosphatase.与大肠杆菌碱性磷酸酶融合的化学合成α-新内啡肽基因的表达
Nucleic Acids Res. 1983 Mar 11;11(5):1283-94. doi: 10.1093/nar/11.5.1283.
3
Expression of cloned beta-endorphin gene sequences by Escherichia coli.大肠杆菌对克隆的β-内啡肽基因序列的表达。
Nature. 1980 Jun 12;285(5765):456-63. doi: 10.1038/285456a0.
4
Leu-enkephalin purification from E. coli cells carrying the plasmid with fused synthetic leu-enkephalin gene.从携带融合合成亮氨酸脑啡肽基因质粒的大肠杆菌细胞中纯化亮氨酸脑啡肽。
Nucleic Acids Res. 1980 Dec 20;8(24):6163-74. doi: 10.1093/nar/8.24.6163.
5
Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor.猪β-新内啡肽/强啡肽前体cDNA的克隆与序列分析
Nature. 1982 Jul 15;298(5871):245-9. doi: 10.1038/298245a0.
6
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.生长抑素激素化学合成基因在大肠杆菌中的表达。
Science. 1977 Dec 9;198(4321):1056-63. doi: 10.1126/science.412251.
7
Expression of cloned calf prochymosin gene sequence in Escherichia coli.克隆的小牛凝乳酶原基因序列在大肠杆菌中的表达。
Gene. 1982 Oct;19(3):337-44. doi: 10.1016/0378-1119(82)90024-5.
8
The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.具有多用途克隆位点的pIC质粒和噬菌体载体，用于通过插入失活进行重组体筛选。
Gene. 1984 Dec;32(3):481-5. doi: 10.1016/0378-1119(84)90022-2.
9
Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6848-52. doi: 10.1073/pnas.80.22.6848.
10
Expression of the human apolipoprotein AI gene fused to the E. coli gene for beta-galactosidase.与大肠杆菌β-半乳糖苷酶基因融合的人载脂蛋白AI基因的表达。
FEBS Lett. 1986 Jan 6;194(2):343-6. doi: 10.1016/0014-5793(86)80114-4.
引用本文的文献
1
Recent developments in the chemical synthesis of polynucleotides.多核苷酸化学合成的最新进展。
Nucleic Acids Res. 1982 Nov 11;10(21):6553-70. doi: 10.1093/nar/10.21.6553.
2
Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.人免疫干扰素化学合成基因在大肠杆菌中的表达
Nucleic Acids Res. 1983 Mar 25;11(6):1707-23. doi: 10.1093/nar/11.6.1707.
3
The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.酵母PHO5基因的核苷酸序列：一种可阻遏酸性磷酸酶的假定前体含有一个信号肽。
Nucleic Acids Res. 1983 Mar 25;11(6):1657-72. doi: 10.1093/nar/11.6.1657.
4
Expression of chemically synthesized alpha-neo-endorphin gene fused to E. coli alkaline phosphatase.与大肠杆菌碱性磷酸酶融合的化学合成α-新内啡肽基因的表达
Nucleic Acids Res. 1983 Mar 11;11(5):1283-94. doi: 10.1093/nar/11.5.1283.
5
A comprehensive list of chemically synthesized genes.化学合成基因的完整列表。
Nucleic Acids Res. 1988 Aug 25;16(16):7763-71. doi: 10.1093/nar/16.16.7763.
本文引用的文献
1
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
The complete amino acid sequence of alpha-neo-endorphin.α-新内啡肽的完整氨基酸序列。
Biochem Biophys Res Commun. 1981 Apr 15;99(3):871-8. doi: 10.1016/0006-291x(81)91244-4.
3
The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips.豚鼠肠道和纵肌条释放的乙酰胆碱的来源。
J Physiol. 1968 Jan;194(1):13-33. doi: 10.1113/jphysiol.1968.sp008392.
4
A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.一种允许真核DNA在细菌中进行调控表达的质粒克隆载体。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3900-4. doi: 10.1073/pnas.73.11.3900.
5
alpha-Neo-endorphin : a "big" Leu-enkephalin with potent opiate activity from porcine hypothalami.α-新内啡肽：一种来自猪下丘脑的具有强效阿片样活性的“大”亮氨酸脑啡肽。
Biochem Biophys Res Commun. 1979 Jan 15;86(1):153-60. doi: 10.1016/0006-291x(79)90394-2.
6
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.生长抑素激素化学合成基因在大肠杆菌中的表达。
Science. 1977 Dec 9;198(4321):1056-63. doi: 10.1126/science.412251.
7
Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.编码人生长激素的DNA序列在大肠杆菌中的直接表达。
Nature. 1979 Oct 18;281(5732):544-8. doi: 10.1038/281544a0.
8
A new method for sequencing DNA.一种新的DNA测序方法。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4. doi: 10.1073/pnas.74.2.560.
9
Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.牛促肾上腺皮质激素-β-促脂素前体克隆cDNA的核苷酸序列
Nature. 1979 Mar 29;278(5703):423-7. doi: 10.1038/278423a0.
10
Expression in Escherichia coli of chemically synthesized genes for human insulin.人胰岛素化学合成基因在大肠杆菌中的表达。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):106-10. doi: 10.1073/pnas.76.1.106.
Zotero 插件