抗核蛋白单克隆抗体对3型小鼠肝炎病毒诱导的急性疾病的保护作用。简报。
Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Brief report.
作者信息
Lecomte J, Cainelli-Gebara V, Mercier G, Mansour S, Talbot P J, Lussier G, Oth D
机构信息
Institut Armand-Frappier, Centre de recherche en virologie, Québec, Canada.
出版信息
Arch Virol. 1987;97(1-2):123-30. doi: 10.1007/BF01310740.
Abstract
Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immunofluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.
摘要
将来自抗MHV-3的A/J小鼠品系的MHV-3免疫脾细胞与NS骨髓瘤细胞融合，产生了几种杂交瘤。在八个杂交瘤克隆中，1E7A4H1克隆分泌κ型IgG2a，显然针对MHV-3病毒粒子的核蛋白。该单克隆抗体能够中和MHV-3对培养的L2细胞的体外细胞病变效应，并通过间接免疫荧光在感染MHV-3的培养YAC细胞上检测到。此外，如果在接种MHV-3之前腹腔注射到C57BL/6小鼠体内，它能对MHV-3诱导的急性疾病提供显著保护。
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