Fasching C E, Peterson L R
Antimicrob Agents Chemother. 1982 Apr;21(4):628-33. doi: 10.1128/AAC.21.4.628.
An anion-exchange column technique for extraction of antibiotics from serum proteins has been developed for use in the assay of cephapirin, cefotaxime, and cefoxitin by high-pressure liquid chromatography. Anion-exchange extraction of cephapirin from serum samples by this technique was compared with protein precipitation methods, using 6% trichloroacetic acid or absolute ethanol. Column extraction gave improved quantitative drug recovery and reduced background serum interferences in the resultant chromatograms when evaluated against protein precipitation. Comparisons of this method with microbiological assay gave statistically equivalent results. Twelve patient samples were assayed for cephapirin, and no interferences were encountered from the 22 systemic agents these subjects were receiving. The anion-exchange technique for antibiotic extraction provides a rapid, precise, and quantitative antibiotic assay when used with liquid chromatography.
已开发出一种用于从血清蛋白中提取抗生素的阴离子交换柱技术，用于通过高压液相色谱法测定头孢匹林、头孢噻肟和头孢西丁。使用6%三氯乙酸或无水乙醇，将该技术从血清样品中阴离子交换提取头孢匹林的方法与蛋白质沉淀法进行了比较。与蛋白质沉淀法相比，柱提取在所得色谱图中提高了药物定量回收率并减少了背景血清干扰。该方法与微生物测定法的比较给出了统计学上等效的结果。对12份患者样品进行了头孢匹林测定，这些受试者正在接受的22种全身用药未产生干扰。当与液相色谱法一起使用时，用于抗生素提取的阴离子交换技术可提供快速、精确和定量的抗生素测定。
