King A M, McCahon D, Slade W R, Newman J W
J Virol. 1982 Jan;41(1):66-77. doi: 10.1128/JVI.41.1.66-77.1982.
Four different pairs of temperature-sensitive mutants, derived from the same strain of aphthovirus, were crossed by using an infectious center recombination test. Each parental mutant carried an unselected marker affecting the isoelectric point of a virus-coded polypeptide; progeny of the crosses, able to grow at the nonpermissive temperature, were screened for these unselected markers. Polypeptide charge was shown to be a stable, inheritable character, and three of the four crosses yielded genetic recombinants that possessed the polypeptide marker from both parents. Peptide fingerprinting and high-resolution isoelectric focusing of the polypeptides ruled out the explantation that these viruses were generated by mutation.
通过感染中心重组试验，对源自同一口蹄疫病毒株的四对不同的温度敏感突变体进行了杂交。每个亲本突变体都携带一个未选择的标记，该标记影响病毒编码多肽的等电点；对能够在非允许温度下生长的杂交后代进行这些未选择标记的筛选。结果表明，多肽电荷是一种稳定的、可遗传的特征，并且四个杂交组合中有三个产生了具有双亲多肽标记的遗传重组体。对这些多肽进行肽指纹图谱分析和高分辨率等电聚焦，排除了这些病毒是由突变产生的解释。
