King A M, McCahon D, Saunders K, Newman J W, Slade W R
Virus Res. 1985 Nov;3(4):373-84. doi: 10.1016/0168-1702(85)90437-x.
Recombinant foot-and-mouth disease viruses were isolated from cells infected with a mixture of temperature-sensitive (ts) mutants belonging to different subtype strains. In order to select for recombination events in many different regions of the genome, crosses were performed between various pairs of mutants, with ts mutations in different regions of the genome. ts+ progeny were analysed by electrofocusing virus-induced proteins and RNase T1 fingerprinting of their RNA. All but 5 out of 43 independent isolates, from nine crosses, proved to have recombinant RNA genomes. Maps of these genomes, based on a knowledge of the locations of the unique oligonucleotides, were constructed. Most could be interpreted as being the products of single genetic cross-overs, although three recombinants were formed by two cross-overs each. Cross-overs in at least twelve distinct regions of the genome were identified. This evidence of a large number of recombination sites suggests that RNA recombination in picornaviruses is a general, as opposed to a site-specific, phenomenon.
从感染了属于不同亚型毒株的温度敏感（ts）突变体混合物的细胞中分离出重组口蹄疫病毒。为了在基因组的许多不同区域选择重组事件，在基因组不同区域带有ts突变的各种突变体对之间进行了杂交。通过电聚焦病毒诱导蛋白和对其RNA进行核糖核酸酶T1指纹分析来分析ts+子代。来自9次杂交的43个独立分离株中，除5个外，其余均被证明具有重组RNA基因组。基于对独特寡核苷酸位置的了解，构建了这些基因组的图谱。大多数可以解释为是单次基因交换的产物，不过有三个重组体是由每次两次交换形成的。在基因组至少十二个不同区域鉴定到了交换。大量重组位点的这一证据表明，小RNA病毒中的RNA重组是一种普遍现象，而非位点特异性现象。
