从克隆的互补DNA产生对胍耐药和依赖的脊髓灰质炎病毒突变体：多肽2C中的突变直接导致胍敏感性改变。
Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity.
作者信息
Pincus S E, Wimmer E
出版信息
J Virol. 1986 Nov;60(2):793-6. doi: 10.1128/JVI.60.2.793-796.1986.
Abstract
cDNA fragments representing the region in polypeptide 2C containing mutations in a guanidine-resistant or -dependent mutant were cloned into the wild-type background of an infectious clone. Transfection of COS-1 cells with these plasmids yielded viruses that were either completely resistant to 2.0 mM guanidine hydrochloride or dependent on this concentration of drug for growth.
摘要
将代表多肽2C中含有胍抗性或依赖性突变区域的cDNA片段克隆到感染性克隆的野生型背景中。用这些质粒转染COS-1细胞产生的病毒，要么对2.0 mM盐酸胍完全抗性，要么生长依赖于该浓度的药物。
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