Leibowitz J L, DeVries J R, Haspel M V
J Virol. 1982 Jun;42(3):1080-7. doi: 10.1128/JVI.42.3.1080-1087.1982.
We performed a genetic analysis of 37 temperature-sensitive mutants of murine hepatitis virus strain JHM. Of our mutants, 32 did not induce murine hepatitis virus-specific RNA synthesis in infected cells at the restrictive temperature, 39 degrees C. By complementation testing we have identified at least seven nonoverlapping complementation groups. Six of the genes identified in this way are required for murine hepatitis virus-specific RNA synthesis. The seventh complementation group is made up of five mutants which induced virus-specific RNA synthesis at 39 degrees C.
我们对鼠肝炎病毒JHM株的37个温度敏感突变体进行了遗传分析。在我们的突变体中，有32个在39℃的限制温度下，不能在受感染细胞中诱导鼠肝炎病毒特异性RNA合成。通过互补试验，我们鉴定出至少7个不重叠的互补群。以这种方式鉴定出的6个基因是鼠肝炎病毒特异性RNA合成所必需的。第七个互补群由5个在39℃时诱导病毒特异性RNA合成的突变体组成。
