大肠杆菌dnaA起始蛋白与dnaZ聚合蛋白在体内的相互作用。
Interaction of the Escherichia coli dnaA initiation protein with the dnaZ polymerization protein in vivo.
作者信息
Walker J R, Ramsey J A, Haldenwang W G
出版信息
Proc Natl Acad Sci U S A. 1982 May;79(10):3340-4. doi: 10.1073/pnas.79.10.3340.
Abstract
To define in vivo interactions of Escherichia coli DNA replication components, extragenic suppressors of a dnaZ(TS) mutant were isolated. A temperature-sensitive dnaZ mutant, which is defective in polymerization, was placed at 39 degrees C to select temperature-insensitive revertants. Some of these revertants also were cold sensitive, a phenotypic property that facilitated study of the suppressor. Mapping of the cold sensitivity indicated that some of the suppressor mutations are intragenic but others are located within the initiation gene, dnaA. The dnaA mutations that suppress the dnaZ(TS) defect are designated dnaA(SUZ, CS). The dnaA(SUZ, CS) strains have a defect in DNA synthesis at low temperature that is typical of an initiation defect. These data suggest that the dnaA product, an initiation factor, interacts in vivo with the dnaZ protein, a polymerization factor.
摘要
为了确定大肠杆菌DNA复制组件的体内相互作用，分离了dnaZ（TS）突变体的基因外抑制子。一个在聚合反应中存在缺陷的温度敏感型dnaZ突变体被置于39℃以选择温度不敏感的回复子。其中一些回复子也是冷敏感型的，这种表型特性便于对抑制子进行研究。冷敏感性的定位表明，一些抑制子突变是基因内的，但其他一些位于起始基因dnaA内。抑制dnaZ（TS）缺陷的dnaA突变被命名为dnaA（SUZ，CS）。dnaA（SUZ，CS）菌株在低温下DNA合成存在缺陷，这是典型的起始缺陷。这些数据表明，作为起始因子的dnaA产物在体内与作为聚合因子的dnaZ蛋白相互作用。
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