产生高表达乙肝表面抗原的重组逆转录病毒DNA。
Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.
作者信息
Stratowa C, Doehmer J, Wang Y, Hofschneider P H
出版信息
EMBO J. 1982;1(12):1573-8. doi: 10.1002/j.1460-2075.1982.tb01357.x.
A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 -124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v-mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.
将编码乙型肝炎病毒表面抗原（HBsAg）的基因组片段插入从鼠细胞系G8 - 124获得的莫洛尼小鼠肉瘤病毒（MSV）的前病毒基因组中。将重组DNA导入NIH 3T3小鼠成纤维细胞。选择由MSV癌基因（v-mosM）进行形态转化的细胞，建立细胞系并检测HBsAg的表达。检测到的表达水平高达4.5微克/10⁷细胞/天。
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