Erisman M D, Linnoila R I, Hernandez O, DiAugustine R P, Lazarus L H
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2379-83. doi: 10.1073/pnas.79.7.2379.
The presence of immunoreactive bombesin in a human lung small-cell carcinoma grown in nude mice was established by several criteria: (i) Radioimmunoassay of tissue extracts for bombesin revealed approximately 6.5 pmol/g of tissue; (ii) bombesin was found in 12-14% of the tumor cells by immunohistochemical localization; (iii) gel filtration of small-cell carcinoma extract on Sephadex G-75 and Bio-Gel P-4 gave only a single peak of immunoreactivity, which occurred at the elution volume of bombesin; and (iv) reverse-phase HPLC of acid-solubilized extracts separated the immunoreactive material into three discrete peaks, one of which eluted with a retention time identical to that of synthetic bombesin. The presence of bombesin may represent the ectopic expression of this peptide in small-cell carcinoma, because immunoreactive bombesin was found in human fetal and neonatal lung but apparently not in adult lung tissue [Wharton, J., Polak, J. M., Bloom, S. R., Ghatei, M. A., Solcia, E., Brown, M. R. & Pearse, A. G. E. (1978) Nature (London) 273, 769-770]. The immunoreactive bombesin previously found in mammalian tissues is considerably larger than amphibian bombesin; these data substantiate the presence of a mammalian form of bombesin in a human tumor that may have a structure similar to that of the amphibian peptide.
（i）对组织提取物进行蛙皮素放射免疫测定，结果显示组织中蛙皮素含量约为6.5 pmol/g；（ii）通过免疫组织化学定位发现12%-14%的肿瘤细胞中存在蛙皮素；（iii）将小细胞癌提取物在Sephadex G-75和Bio-Gel P-4上进行凝胶过滤，仅得到一个免疫反应性单峰，其洗脱体积与蛙皮素的洗脱体积一致；（iv）对酸溶性提取物进行反相高效液相色谱分析，将免疫反应性物质分离为三个离散峰，其中一个峰的洗脱保留时间与合成蛙皮素相同。蛙皮素的存在可能代表该肽在小细胞癌中的异位表达，因为在人胎儿和新生儿肺中发现了免疫反应性蛙皮素，但在成人肺组织中显然未发现[沃顿，J.，波拉克，J. M.，布卢姆，S. R.，加泰，M. A.，索尔恰，E., 布朗，M. R. & 皮尔斯，A. G. E.（1978年）《自然》（伦敦）273, 769 - 770]。先前在哺乳动物组织中发现的免疫反应性蛙皮素比两栖类蛙皮素大得多；这些数据证实了在人类肿瘤中存在一种哺乳动物形式的蛙皮素，其结构可能与两栖类肽的结构相似。
