Sakaguchi A Y, Shows T B
Somatic Cell Genet. 1982 Jan;8(1):83-94. doi: 10.1007/BF01538652.
Human coronavirus 229E, n enveloped, RNA-containing virus, causes respiratory illness in man and is serologically related to murine coronavirus JHM, which causes acute and chronic demyelination in rodents. 229E displays a species-specific host range restriction whose genetic basis was studied in human-mouse hybrids. 229E replicated in human WI-38 cells but not in three mouse cell lines tested (RAG, LM/TK-, and A9). Human coronavirus sensitivity (HCVS) was expressed as a dominant phenotype in hybrids, indicating that mouse cells do not actively suppress 229E replication. HCVS segregated concordantly with the human chromosome 15 enzyme markers mannose phosphate isomerase (MPI) and the muscle form of pyruvate kinase (PKM2), and analysis of hybrids containing an X/15 translocation [t(X;15)(p11;q11)] localized HCVS to the q11 leads to qter region of chromosome 15. HCVS might code for a specific surface receptor, allowing 229E to be absorbed to and received within the host cell.
人冠状病毒229E是一种有包膜、含RNA的病毒,可引起人类呼吸道疾病,在血清学上与鼠冠状病毒JHM相关,后者可导致啮齿动物出现急性和慢性脱髓鞘。229E表现出物种特异性的宿主范围限制,其遗传基础在人-鼠杂交细胞中进行了研究。229E能在人WI-38细胞中复制,但在三种测试的小鼠细胞系(RAG、LM/TK-和A9)中不能复制。人冠状病毒敏感性(HCVS)在杂交细胞中表现为显性表型,这表明小鼠细胞不会主动抑制229E的复制。HCVS与人类15号染色体酶标记物磷酸甘露糖异构酶(MPI)和肌肉型丙酮酸激酶(PKM2)呈一致分离,对含有X/15易位[t(X;15)(p11;q11)]的杂交细胞进行分析,将HCVS定位于15号染色体q11至qter区域。HCVS可能编码一种特异性表面受体,使229E能够被宿主细胞吸附并进入细胞内。