通过基因重配进行不可培养的人轮状病毒的拯救及血清型鉴定

Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.

作者信息

Greenberg H B, Wyatt R G, Kapikian A Z, Kalica A R, Flores J, Jones R

出版信息

Infect Immun. 1982 Jul;37(1):104-9. doi: 10.1128/iai.37.1.104-109.1982.

DOI:10.1128/iai.37.1.104-109.1982

PMID:6286486

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347496/

Abstract

Thirty-three of 50 noncultivable human rotavirus strains from a variety of locations were successfully rescued by gene reassortment. The serotype of each of the 33 strains was investigated by a qualitative cytopathic effect neutralization assay. Nineteen strains resembled the previously characterized human rotavirus serotype Wa, whereas three strains were serologically related to the DS-1 strain. Eleven strains appeared to be serotypically distinct from the Wa and DS-1 strains and thus apparently represent one or more new human rotavirus serotypes.

摘要

从不同地点采集的50株不可培养的人类轮状病毒株中，有33株通过基因重配成功获救。通过定性细胞病变效应中和试验对这33株病毒株中的每一株血清型进行了研究。19株病毒株与先前鉴定的人类轮状病毒血清型Wa相似，而3株病毒株与DS-1株血清学相关。11株病毒株似乎在血清型上与Wa株和DS-1株不同，因此显然代表一种或多种新的人类轮状病毒血清型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a3f/347496/c0a68f59570c/iai00148-0117-a.jpg

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通过基因重配进行不可培养的人轮状病毒的拯救及血清型鉴定

Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment.

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