铜绿假单胞菌的转化与转染：金属离子的影响
Transformation and transfection of Pseudomonas aeruginosa: effects of metal ions.
作者信息
Mercer A A, Loutit J S
出版信息
J Bacteriol. 1979 Oct;140(1):37-42. doi: 10.1128/jb.140.1.37-42.1979.
Abstract
The ability of different metal ions to promote transformation of Pseudomonas aeruginosa by deoxyribonucleic acid of the plasmid RP1 was examined. CaCl2, MgCl2, and MnCl2 were found to promote such transformation, although at different frequencies and with the optimum response at different concentrations. Only MgCl2 promoted transfection of P. aeruginosa by the linear deoxyribonucleic acid of phage F116. CaCl2 was demonstrated to allow adsorption and entry into the cell of F116 deoxyribonucleic acid such that it became resistant to exogenous deoxyribonuclease, but phage production occurred only when MgCl2 was provided. Inactivation of linear phage deoxyribonucleic acid taken up in the absence of MgCl2 was observed. The transfection frequencies at various concentrations of MgCl2 were compared, and the optimum response occurred at the concentration which promoted the highest frequency of transformation by RP1 deoxyribonucleic acid.
摘要
研究了不同金属离子通过质粒RP1的脱氧核糖核酸促进铜绿假单胞菌转化的能力。发现氯化钙、氯化镁和氯化锰可促进这种转化，尽管频率不同且在不同浓度下有最佳反应。只有氯化镁能促进噬菌体F116的线性脱氧核糖核酸对铜绿假单胞菌的转染。已证明氯化钙能使F116脱氧核糖核酸吸附并进入细胞，使其对外源脱氧核糖核酸酶具有抗性，但只有在提供氯化镁时才会产生噬菌体。观察到在没有氯化镁的情况下摄取的线性噬菌体脱氧核糖核酸会失活。比较了不同浓度氯化镁下的转染频率，最佳反应出现在促进RP1脱氧核糖核酸转化频率最高的浓度下。
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