Acker J, Wintzerith M, Vigneron M, Kedinger C
Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 de Biologie Moléculaire et de Génie Génétique (INSERM), Institut de Chimie Biologique, Strasbourg, France.
Nucleic Acids Res. 1993 Nov 25;21(23):5345-50. doi: 10.1093/nar/21.23.5345.
The structure of the gene encoding the 14.5 kDa subunit of the human RNA polymerase II (or B) has been elucidated. The gene consists of six exons, ranging from 52 to over 101 bp, interspaced with five introns ranging from 84 to 246 bp. It is transcribed into three major RNA species, present at low abundance in exponentially growing HeLa cells. The corresponding messenger RNAs contain the same open reading frame encoding a 125 amino acid residue protein, with a calculated molecular weight of 14,523 Da. This protein (named hRPB14.5) shares strong homologies with the homologous polymerase subunits encoded by the Drosophila (RpII15) and yeast (RPB9) genes. Cysteines characteristic of two zinc fingers are conserved in all three corresponding sequences and, like the yeast protein, the hRPB14.5 subunit exhibits zinc-binding activity.
编码人RNA聚合酶II（或B）14.5 kDa亚基的基因结构已被阐明。该基因由六个外显子组成，长度从52 bp到超过101 bp不等，中间间隔着五个内含子，长度从84 bp到246 bp不等。它转录成三种主要的RNA种类，在指数生长的HeLa细胞中含量较低。相应的信使RNA包含相同的开放阅读框，编码一个125个氨基酸残基的蛋白质，计算分子量为14,523 Da。这种蛋白质（命名为hRPB14.5）与果蝇（RpII15）和酵母（RPB9）基因编码的同源聚合酶亚基具有很强的同源性。两个锌指特有的半胱氨酸在所有三个相应序列中都是保守的，并且与酵母蛋白一样，hRPB14.5亚基表现出锌结合活性。
