Dugard J, Kew M C, da Fonseca M, Levin J
S Afr Med J. 1982 Aug 21;62(9):271-3.
Having found raised serum calcitonin concentrations in 94% of patients with hepatocellular carcinoma when using a dextran-coated-charcoal radio-immunoassay, we have now repeated the study, using a double-antibody radio-immunoassay, in 102 further patients with hepatocellular carcinoma and 35 matched controls. Serum immunoreactive calcitonin concentrations (iCT) in the controls ranged from 10 to 310 pg/ml (mean 154.6 pg/ml). Values in the tumour patients ranged from 10 to 1,650 pg/ml (mean 302.6 pg/ml). The mean figures were significantly higher in the tumour patients (P less than 0.001), 35.5% of them having values above 310 pg/ml. In 65 of the patients serum iCT concentrations were also determined by dextran-coated-charcoal radio-immunoassay. Values ranged from 10 to 10,780 pg/ml (mean 2,179 pg/ml). If 1,000 pg/ml is taken as the upper limit of normal, 69% of the patients had raised iCT concentrations. There was a good correlation (r = 0.67; P less than 0.001) between serum iCT values measured with both methods in 50 patients. If measured by the double-antibody radio-immunoassay method, the serum calcitonin value is not useful as a marker for hepatocellular carcinoma.
我们在采用葡聚糖包被活性炭放射免疫分析法时发现，94%的肝细胞癌患者血清降钙素浓度升高。现在，我们采用双抗体放射免疫分析法，对另外102例肝细胞癌患者和35例配对对照者进行了重复研究。对照组血清免疫反应性降钙素浓度（iCT）为10至310 pg/ml（平均154.6 pg/ml）。肿瘤患者的值为10至1650 pg/ml（平均302.6 pg/ml）。肿瘤患者的平均数值显著更高（P<0.001），其中35.5%的值高于310 pg/ml。在65例患者中，还采用葡聚糖包被活性炭放射免疫分析法测定了血清iCT浓度。值为10至10780 pg/ml（平均2179 pg/ml）。若将1000 pg/ml作为正常上限，则69%的患者iCT浓度升高。50例患者中，两种方法测得的血清iCT值之间存在良好的相关性（r = 0.67；P<0.001）。若采用双抗体放射免疫分析法测量，血清降钙素值作为肝细胞癌的标志物并无用处。
