Treuhaft M W, Beem M O
Infect Immun. 1982 Aug;37(2):439-44. doi: 10.1128/iai.37.2.439-444.1982.
A multiplicity-dependent interference was observed in respiratory syncytial virus preparations (Randall strain) grown in HEp-2 cells, and the factor mediating this interference was characterized. Cloned virus did not demonstrate this multiplicity-dependent interference, but its replication was shown to be inhibited by the interfering factor by assays of reduction of infectious yield assay, the interferon factor was found to be particulate, to be inactivated by UV irradiation, and not to interfere with the replication of a heterologous virus, vesicular stomatitis virus. These characteristics are compatible with the physical properties and biological behavior of defective interfering particles. Defective interfering particles were generated by four undiluted passages of cloned virus but were not apparent after eight passages at a multiplicity of infection of 0.1.
在人喉上皮细胞（HEp-2细胞）中培养的呼吸道合胞病毒制剂（兰德尔毒株）中观察到了一种多重依赖性干扰现象，并对介导这种干扰的因子进行了特性鉴定。克隆病毒未表现出这种多重依赖性干扰，但其复制通过感染性产量降低试验被证明会受到干扰因子的抑制。干扰素因子被发现是颗粒状的，能被紫外线照射灭活，且不干扰异源病毒水疱性口炎病毒的复制。这些特性与缺陷干扰颗粒的物理性质和生物学行为相符。通过克隆病毒的四次未稀释传代产生了缺陷干扰颗粒，但在感染复数为0.1的情况下传代八次后，缺陷干扰颗粒并不明显。
