Bergeron J J, Kotwal G J, Levine G, Bilan P, Rachubinski R, Hamilton M, Shore G C, Ghosh H P
J Cell Biol. 1982 Jul;94(1):36-41. doi: 10.1083/jcb.94.1.36.
The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitive to endoglycosidase H. Silver grains were subsequently (at 30-40 min) observed over the Golgi apparatus, and the [3H]mannose-labeled G protein became resistant to endoglycosidase H digestion. Our data directly demonstrate the intracellular transport of a plasmalemma-destined transmembrane glycoprotein through the Golgi apparatus.
在用[3H]甘露糖脉冲处理2分钟后,接着进行不同时间间隔的非放射性追踪,通过光学显微镜和电子显微镜放射自显影观察水泡性口炎病毒感染细胞中G蛋白的细胞内迁移。放射性最初(在5 - 10分钟时)主要出现在内质网中,产生的[3H]甘露糖标记的G蛋白对内切糖苷酶H敏感。随后(在30 - 40分钟时)在高尔基体上观察到银颗粒,并且[3H]甘露糖标记的G蛋白对内切糖苷酶H消化变得有抗性。我们的数据直接证明了一种注定要到达质膜的跨膜糖蛋白通过高尔基体进行细胞内运输。