通过电子显微镜放射自显影观察水泡性口炎病毒跨膜糖蛋白G在高尔基器中的细胞内运输。
Intracellular transport of the transmembrane glycoprotein G of vesicular stomatitis virus through the Golgi apparatus as visualized by electron microscope radioautography.
作者信息
Bergeron J J, Kotwal G J, Levine G, Bilan P, Rachubinski R, Hamilton M, Shore G C, Ghosh H P
出版信息
J Cell Biol. 1982 Jul;94(1):36-41. doi: 10.1083/jcb.94.1.36.
Abstract
The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitive to endoglycosidase H. Silver grains were subsequently (at 30-40 min) observed over the Golgi apparatus, and the [3H]mannose-labeled G protein became resistant to endoglycosidase H digestion. Our data directly demonstrate the intracellular transport of a plasmalemma-destined transmembrane glycoprotein through the Golgi apparatus.
摘要
在用[3H]甘露糖脉冲处理2分钟后，接着进行不同时间间隔的非放射性追踪，通过光学显微镜和电子显微镜放射自显影观察水泡性口炎病毒感染细胞中G蛋白的细胞内迁移。放射性最初（在5 - 10分钟时）主要出现在内质网中，产生的[3H]甘露糖标记的G蛋白对内切糖苷酶H敏感。随后（在30 - 40分钟时）在高尔基体上观察到银颗粒，并且[3H]甘露糖标记的G蛋白对内切糖苷酶H消化变得有抗性。我们的数据直接证明了一种注定要到达质膜的跨膜糖蛋白通过高尔基体进行细胞内运输。
相似文献
1
Intracellular transport of the transmembrane glycoprotein G of vesicular stomatitis virus through the Golgi apparatus as visualized by electron microscope radioautography.通过电子显微镜放射自显影观察水泡性口炎病毒跨膜糖蛋白G在高尔基器中的细胞内运输。
J Cell Biol. 1982 Jul;94(1):36-41. doi: 10.1083/jcb.94.1.36.
2
Transport of protein between cytoplasmic membranes of fused cells: correspondence to processes reconstituted in a cell-free system.融合细胞胞质膜间蛋白质的转运：与无细胞体系中重组过程的对应关系。
J Cell Biol. 1984 Jul;99(1 Pt 1):248-59. doi: 10.1083/jcb.99.1.248.
3
Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.包被小泡分两个连续阶段将新合成的膜糖蛋白从内质网运输到质膜。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):780-4. doi: 10.1073/pnas.77.2.780.
4
Immunoelectron microscopic studies of the intracellular transport of the membrane glycoprotein (G) of vesicular stomatitis virus in infected Chinese hamster ovary cells.水泡性口炎病毒膜糖蛋白（G）在感染的中国仓鼠卵巢细胞内运输的免疫电子显微镜研究。
J Cell Biol. 1983 Dec;97(6):1777-87. doi: 10.1083/jcb.97.6.1777.
5
Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells.水泡性口炎病毒新合成的G蛋白在有丝分裂细胞中不会转运至高尔基体复合体。
J Cell Biol. 1985 Dec;101(6):2036-46. doi: 10.1083/jcb.101.6.2036.
6
Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.一种整合膜蛋白，即水泡性口炎病毒糖蛋白，在前往质膜的途中穿过高尔基体。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1746-50. doi: 10.1073/pnas.78.3.1746.
7
Involvement of Golgi apparatus and a restructured nuclear envelope during biogenesis and transport of herpes simplex virus glycoproteins.单纯疱疹病毒糖蛋白生物合成与运输过程中高尔基体和重组核膜的参与。
J Histochem Cytochem. 1985 Sep;33(9):875-83. doi: 10.1177/33.9.2991363.
8
The morphologic pathway of exocytosis of the vesicular stomatitis virus G protein in cultured fibroblasts.水泡性口炎病毒G蛋白在培养的成纤维细胞中胞吐作用的形态学途径。
Cell. 1982 Apr;28(4):831-41. doi: 10.1016/0092-8674(82)90062-9.
9
Transport of the membrane glycoprotein of vesicular stomatitis virus to the cell surface in two stages by clathrin-coated vesicles.水泡性口炎病毒膜糖蛋白通过网格蛋白包被小泡分两个阶段转运至细胞表面。
J Cell Biol. 1980 Jul;86(1):162-71. doi: 10.1083/jcb.86.1.162.
10
Intracellular transport and processing of Sindbis virus glycoproteins.辛德毕斯病毒糖蛋白的细胞内运输与加工
Virology. 1989 May;170(1):117-22. doi: 10.1016/0042-6822(89)90358-9.
引用本文的文献
1
Chapter 6 Protein Sorting in the Secretory Pathway.第6章 分泌途径中的蛋白质分选
Curr Top Membr Transp. 1985;24:251-294. doi: 10.1016/S0070-2161(08)60328-7. Epub 2008 May 30.
2
Isolation and compositional analysis of secretion granules and their membrane subfraction from the rat parotid gland.大鼠腮腺分泌颗粒及其膜亚组分的分离与成分分析。
J Membr Biol. 1984;79(2):127-44. doi: 10.1007/BF01872117.
3
Intracellular transport of influenza virus hemagglutinin to the apical surface of Madin-Darby canine kidney cells.流感病毒血凝素在细胞内运输至犬肾传代细胞（Madin-Darby canine kidney cells）顶端表面的过程。
J Cell Biol. 1984 Jan;98(1):308-19. doi: 10.1083/jcb.98.1.308.
4
Intracellular vesicles involved in the transport of Semliki Forest virus membrane proteins to the cell surface.参与将塞姆利基森林病毒膜蛋白转运至细胞表面的细胞内囊泡。
EMBO J. 1983;2(11):2001-6. doi: 10.1002/j.1460-2075.1983.tb01691.x.
5
Somatostatin discriminates between the intracellular pathways of secretory and membrane proteins.生长抑素可区分分泌蛋白和膜蛋白的细胞内运输途径。
J Cell Biol. 1984 Jul;99(1 Pt 1):97-104. doi: 10.1083/jcb.99.1.97.
6
Biogenesis of the polymeric IgA receptor in rat hepatocytes. II. Localization of its intracellular forms by cell fractionation studies.大鼠肝细胞中聚合免疫球蛋白A受体的生物发生。II. 通过细胞分级分离研究对其细胞内形式进行定位
J Cell Biol. 1985 Apr;100(4):1255-61. doi: 10.1083/jcb.100.4.1255.
7
Intracellular assembly and packaging of hepatitis B surface antigen particles occur in the endoplasmic reticulum.乙型肝炎表面抗原颗粒的细胞内组装和包装发生在内质网中。
J Virol. 1986 Jun;58(3):884-92. doi: 10.1128/JVI.58.3.884-892.1986.
8
The G protein of vesicular stomatitis virus has free access into and egress from the smooth endoplasmic reticulum of UT-1 cells.水疱性口炎病毒的G蛋白可自由进出UT-1细胞的光滑内质网。
J Cell Biol. 1990 Mar;110(3):625-35. doi: 10.1083/jcb.110.3.625.
本文引用的文献
1
Synthesis and maturation of glycoproteins of enveloped animal viruses.包膜动物病毒糖蛋白的合成与成熟
Rev Infect Dis. 1980 Jan-Feb;2(1):26-39. doi: 10.1093/clinids/2.1.26.
2
Distribution of terminal glycosyltransferases in hepatic Golgi fractions.末端糖基转移酶在肝高尔基体组分中的分布。
J Cell Biol. 1980 Jan;84(1):87-101. doi: 10.1083/jcb.84.1.87.
3
Passage of an integral membrane protein, the vesicular stomatitis virus glycoprotein, through the Golgi apparatus en route to the plasma membrane.一种整合膜蛋白，即水泡性口炎病毒糖蛋白，在前往质膜的途中穿过高尔基体。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1746-50. doi: 10.1073/pnas.78.3.1746.
4
Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.包被小泡分两个连续阶段将新合成的膜糖蛋白从内质网运输到质膜。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):780-4. doi: 10.1073/pnas.77.2.780.
5
Formation of lipid-bound oligosaccharides containing mannose. Their role in glycoprotein synthesis.含甘露糖的脂质结合寡糖的形成。它们在糖蛋白合成中的作用。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3390-4. doi: 10.1073/pnas.70.12.3390.
6
Synthesis in vitro of vesicular stomatitis virus proteins in cytoplasmic extracts of L cells.在L细胞的细胞质提取物中体外合成水疱性口炎病毒蛋白。
Biochem Biophys Res Commun. 1973 Sep 5;54(1):228-33. doi: 10.1016/0006-291x(73)90912-1.
7
Intracellular aspects of the process of protein synthesis.蛋白质合成过程的细胞内层面
Science. 1975 Aug 1;189(4200):347-58. doi: 10.1126/science.1096303.
8
Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked oligosaccharides.一种能够加工天冬酰胺连接寡糖的大鼠肝脏高尔基体α-甘露糖苷酶的纯化与特性分析
J Biol Chem. 1979 Nov 25;254(22):11655-63.
9
Binding and uptake of 125I-insulin into rat liver hepatocytes and endothelium. An in vivo radioautographic study.125I标记胰岛素在大鼠肝脏肝细胞和内皮细胞中的结合与摄取。一项体内放射自显影研究。
J Cell Biol. 1979 Feb;80(2):427-43. doi: 10.1083/jcb.80.2.427.
10
Polypeptide hormone binding sites in vivo: initial localization of 125I-labeled insulin to hepatocyte plasmalemma as visualized by electron microscope radioautography.体内的多肽激素结合位点：通过电子显微镜放射自显影观察到，125I标记的胰岛素在肝细胞质膜上的初始定位。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5051-5. doi: 10.1073/pnas.74.11.5051.
Zotero 插件