Sorenson G D, Bloom S R, Ghatei M A, Del Prete S A, Cate C C, Pettengill O S
Regul Pept. 1982 Jul;4(2):59-66. doi: 10.1016/0167-0115(82)90095-7.
A series of continuous cell lines of human small cell carcinoma of the lung (SCCL) have been evaluated for the production of bombesin (BN). In early established cultures BN was detected in the medium of 9 out of 11 cell lines and in 6 out of 7 cell homogenates examined. Levels in the medium were frequently higher in cultures of later passages compared to earlier passages of the same line and low levels developed in the two previously negative cell lines. Plasma concentrations were greater than 80 pmol/l in 2 out of 27 (7%) randomly selected patients with SCCL. A culture (DMS 406) established from the tumor of a patient with the highest plasma level (1240 pmol/l) was the highest producer in vitro. The results indicate that BN, which has been demonstrated immunocytochemically to be present in normal bronchial mucosal cells, is frequently produced by SCCL in vitro but elevated plasma levels are infrequently found in patients with this neoplasm.
已对一系列人肺小细胞癌（SCCL）连续细胞系进行了蛙皮素（BN）产生情况的评估。在早期建立的培养物中，在检测的11个细胞系中的9个细胞系的培养基以及7个细胞匀浆中的6个中检测到了BN。与同一细胞系的早期传代培养相比，后期传代培养的培养基中的水平通常更高，并且在两个先前呈阴性的细胞系中出现了低水平。在27例随机选择的SCCL患者中，有2例（7%）的血浆浓度大于80 pmol/l。从血浆水平最高（1240 pmol/l）的患者肿瘤中建立的一种培养物（DMS 406）是体外产量最高的。结果表明，免疫细胞化学已证明正常支气管黏膜细胞中存在BN，SCCL在体外经常产生BN，但该肿瘤患者中血浆水平升高的情况并不常见。
