Lackie P M, Cuttitta F, Minna J D, Bloom S R, Polak J M
Histochemistry. 1985;83(1):57-9. doi: 10.1007/BF00495300.
The role of regulatory peptides and the existence of specific peptide receptors are becoming established. However, techniques for the ultrastructural localisation of these receptors are fraught with difficulties. We propose here a novel technique for receptor localisation using a dimeric peptide ligand and electron microscopical immunocytochemistry. The dimeric ligand is used as a bridge between the receptor and a specific anti-ligand antibody. By this method we have localised receptors for bombesin in cells of small cell lung carcinoma in culture. The results support previous biochemical evidence for the existence of said receptors and the technique should be applicable for the localisation of other receptors recognising small ligands.
调节肽的作用和特定肽受体的存在正逐渐得到确认。然而，对这些受体进行超微结构定位的技术却充满困难。我们在此提出一种利用二聚体肽配体和电子显微镜免疫细胞化学进行受体定位的新技术。二聚体配体用作受体与特异性抗配体抗体之间的桥梁。通过这种方法，我们已在培养的小细胞肺癌细胞中定位了蛙皮素受体。这些结果支持了先前关于所述受体存在的生化证据，并且该技术应适用于定位识别小分子配体的其他受体。
