Rutanen E M, Bohn H, Seppälä M
Am J Obstet Gynecol. 1982 Oct 15;144(4):460-3. doi: 10.1016/0002-9378(82)90254-x.
A radioimmunoassay for placental protein 12 (PP12) is described and the levels in amniotic fluid, cord blood, and serum of nonpregnant individuals, pregnant women, and patients with trophoblastic disease are presented. During pregnancy, the highest PP12 levels were found at 22 to 23 weeks (mean +/- SD, 169 +/- 123 ng/ml), and there was a transient decline at 32 to 33 weeks (63 +/- 23 ng/ml). In amniotic fluid, the levels were 100 to 1,000 times higher than in maternal serum. In cord blood at birth, the values were of the same magnitude as in maternal serum. Also healthy nonpregnant women and men had PP12-like immunoreactivity in serum. Nonpregnant women (9 to 47 ng/ml) had higher levels than men (undetectable to 21 ng/ml). Elevated levels up to 84 ng/ml were occasionally observed in trophoblastic disease, both hydatidiform mole and choriocarcinoma, but they bore no correlation with the human chorionic gonadotropin levels. On the basis of these results PP12 is not a suitable marker for trophoblastic disease. PP12 values in normal pregnancy provide the basis for the evaluation of PP12 levels in abnormal pregnancy.
本文描述了一种用于检测胎盘蛋白12（PP12）的放射免疫分析方法，并给出了非妊娠个体、孕妇以及滋养层疾病患者羊水、脐血和血清中的PP12水平。在孕期，PP12水平在22至23周时最高（均值±标准差，169±123 ng/ml），在32至33周时出现短暂下降（63±23 ng/ml）。羊水中的PP12水平比母血血清中的高100至1000倍。出生时脐血中的PP12水平与母血血清中的相当。健康的非妊娠女性和男性血清中也有PP12样免疫反应性。非妊娠女性（9至47 ng/ml）的PP12水平高于男性（检测不到至21 ng/ml）。在滋养层疾病（包括葡萄胎和绒毛膜癌）中偶尔观察到PP12水平升高至84 ng/ml，但它们与人类绒毛膜促性腺激素水平无关。基于这些结果，PP12不是滋养层疾病的合适标志物。正常妊娠中的PP12值为评估异常妊娠中的PP12水平提供了依据。
