Poklis A, Mackell M A
Clin Chem. 1982 Oct;28(10):2125-7.
We evaluated a new alcohol dehydrogenase (EC 1.1.1.1) enzymic assay (ADH-glycine, Sigma Chemical Co.) for the determination of ethanol in blood. This assay differs from the manufacturer's previous assay (ADH-pyrophosphate) in that glycine replaces pyrophosphate as the buffer and hydrazine replaces semicarbazide as the trapping agent. The standard curve for the assay was linear over blood ethanol concentrations of 0.50-5.00 g/L. The reaction time of the assay was 10 min. At 1.00 g/L within-run and between-run CVs were 3.96% (n = 20) and 4.01% (n = 20), respectively. Mean analytical recovery of ethanol added to whole blood at 0.50-5.00 g/L was 99.7% (SD 2.6%). We performed 100 consecutive clinical and forensic determinations by the ADH-glycine assay, the ADH-pyrophosphate assay, and gas chromatography. Correlation coefficients of the results by least-square linear regression were 0.995 for ADH-pyrophosphate vs ADH-glycine, and 0.990 for gas chromatography vs ADH-glycine. The major advantage of the ADH-glycine assay over the ADH-pyrophosphate assay is the shorter reaction time, 10 min vs 30 min.
我们评估了一种用于测定血液中乙醇的新型乙醇脱氢酶（EC 1.1.1.1）酶法检测（ADH-甘氨酸法，西格玛化学公司）。该检测方法与制造商先前的检测方法（ADH-焦磷酸法）不同之处在于，甘氨酸取代焦磷酸作为缓冲剂，肼取代氨基脲作为捕获剂。该检测方法的标准曲线在血液乙醇浓度为0.50 - 5.00 g/L范围内呈线性。该检测方法的反应时间为10分钟。在1.00 g/L时，批内和批间变异系数分别为3.96%（n = 20）和4.01%（n = 20）。添加到全血中的乙醇在0.50 - 5.00 g/L时的平均分析回收率为99.7%（标准差2.6%）。我们通过ADH-甘氨酸法、ADH-焦磷酸法和气相色谱法连续进行了100次临床和法医检测。通过最小二乘线性回归得到的结果的相关系数，ADH-焦磷酸法与ADH-甘氨酸法为0.995，气相色谱法与ADH-甘氨酸法为0.990。ADH-甘氨酸法相对于ADH-焦磷酸法的主要优点是反应时间更短，分别为10分钟和30分钟。
