文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Goto Y, Amuro N, Ohki Y, Shukuya R

Comp Biochem Physiol B. 1982;72(4):637-40. doi: 10.1016/0305-0491(82)90518-1.

The heterogeneity of liver mitochondria of the tadpoles, Rana catesbeiana, undergoing metamorphosis was investigated by a combination of pulse-chase labeling of mitochondria with [methyl-3H]thymidine and centrifugation of mitochondria on a density gradient of metrizamide. 2. The liver mitochondria of tadpole at premetamorphic stage are separated into two populations with mean densities of 1.128 (M2) and 1.112 (M3). 3. At metamorphic stage a population with mean densities of 1.174 (M1) appears additionally. 4. The activity of mitochondria to take up [methyl-3H]thymidine in vivo is 2-3 times higher at metamorphic stage than at premetamorphic stage. 5. The M1 population has a prominently high activity to take up L-[4.5-3H]leucine in vitro and also a high specific activity of cytochrome c oxidase. 6. These findings suggest that the mitochondrial populations found are of alternate stages in the mitochondrial maturation.

通过用[甲基-³H]胸腺嘧啶核苷对线粒体进行脉冲追踪标记并在甲泛影酰胺密度梯度上对线粒体进行离心相结合的方法，研究了正经历变态的牛蛙蝌蚪肝脏线粒体的异质性。2. 变态前阶段蝌蚪的肝脏线粒体被分为两个群体，平均密度分别为1.128（M2）和1.112（M3）。3. 在变态阶段，额外出现了一个平均密度为1.174（M1）的群体。4. 线粒体在体内摄取[甲基-³H]胸腺嘧啶核苷的活性在变态阶段比变态前阶段高2至3倍。5. M1群体在体外摄取L-[4.5-³H]亮氨酸的活性显著较高，并且细胞色素c氧化酶的比活性也很高。6. 这些发现表明，所发现的线粒体群体处于线粒体成熟的不同阶段。

Goto Y, Amuro N, Ohki Y, Shukuya R

Comp Biochem Physiol B. 1982;72(4):637-40. doi: 10.1016/0305-0491(82)90518-1.

The heterogeneity of liver mitochondria of the tadpoles, Rana catesbeiana, undergoing metamorphosis was investigated by a combination of pulse-chase labeling of mitochondria with [methyl-3H]thymidine and centrifugation of mitochondria on a density gradient of metrizamide. 2. The liver mitochondria of tadpole at premetamorphic stage are separated into two populations with mean densities of 1.128 (M2) and 1.112 (M3). 3. At metamorphic stage a population with mean densities of 1.174 (M1) appears additionally. 4. The activity of mitochondria to take up [methyl-3H]thymidine in vivo is 2-3 times higher at metamorphic stage than at premetamorphic stage. 5. The M1 population has a prominently high activity to take up L-[4.5-3H]leucine in vitro and also a high specific activity of cytochrome c oxidase. 6. These findings suggest that the mitochondrial populations found are of alternate stages in the mitochondrial maturation.

通过用[甲基-³H]胸腺嘧啶核苷对线粒体进行脉冲追踪标记并在甲泛影酰胺密度梯度上对线粒体进行离心相结合的方法，研究了正经历变态的牛蛙蝌蚪肝脏线粒体的异质性。2. 变态前阶段蝌蚪的肝脏线粒体被分为两个群体，平均密度分别为1.128（M2）和1.112（M3）。3. 在变态阶段，额外出现了一个平均密度为1.174（M1）的群体。4. 线粒体在体内摄取[甲基-³H]胸腺嘧啶核苷的活性在变态阶段比变态前阶段高2至3倍。5. M1群体在体外摄取L-[4.5-³H]亮氨酸的活性显著较高，并且细胞色素c氧化酶的比活性也很高。6. 这些发现表明，所发现的线粒体群体处于线粒体成熟的不同阶段。