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大肠杆菌dnaW突变是adk基因的一个等位基因。

The Escherichia coli dnaW mutation is an allele of the adk gene.

作者信息

Henson J M, Blinkowa A, Walker J R

出版信息

Mol Gen Genet. 1982;186(4):488-92. doi: 10.1007/BF00337953.

DOI:10.1007/BF00337953
PMID:6290847
Abstract

A dnaW mutant, isolated on the basis of inability to effect conjugal DNA transfer at high temperature, has been shown by complementation and enzyme assay to be defective in the adk (adenylate kinase; EC 2.7.4.3) locus. The adk mutant, known to have reduced ATP concentration at the nonpermissive temperature (Cousin and Belaich 1966), was used to demonstrate a donor energy requirement for stable aggregate formation and for chromosome transfer in conjugation.

摘要

一个基于在高温下无法进行接合性DNA转移而分离出的dnaW突变体,通过互补和酶测定已表明在adk(腺苷酸激酶;EC 2.7.4.3)位点存在缺陷。已知该adk突变体在非允许温度下ATP浓度降低(Cousin和Belaich,1966),被用于证明供体能量对于稳定聚集体形成和接合中染色体转移的需求。

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1
The Escherichia coli dnaW mutation is an allele of the adk gene.大肠杆菌dnaW突变是adk基因的一个等位基因。
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2
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Cloning and sequencing of the adenylate kinase gene (adk) of Escherichia coli.大肠杆菌腺苷酸激酶基因(adk)的克隆与测序
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Adenylate kinases from thermosensitive Escherichia coli strains.来自温度敏感型大肠杆菌菌株的腺苷酸激酶
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Identification and purification of an adenylate kinase-associated protein that influences the thermolability of adenylate kinase from a temperature-sensitive adk mutant of Escherichia coli.从大肠杆菌的温度敏感型腺苷酸激酶(adk)突变体中鉴定并纯化一种影响腺苷酸激酶热稳定性的腺苷酸激酶相关蛋白。
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引用本文的文献

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Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
2
Isolation and characterization of adenylate kinase (adk) mutations in Salmonella typhimurium which block the ability of glycine betaine to function as an osmoprotectant.鼠伤寒沙门氏菌中腺苷酸激酶(adk)突变的分离与鉴定,这些突变阻断了甘氨酸甜菜碱作为渗透保护剂发挥作用的能力。
J Bacteriol. 1995 Jan;177(2):390-400. doi: 10.1128/jb.177.2.390-400.1995.
3
Linkage map of Escherichia coli K-12, edition 7.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.一个控制大肠杆菌K-12中丝状形成和对辐射敏感性的基因座。
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Genetic analysis of Escherichia coli mutants defective in adenylate kinase and sn-glycerol 3-phosphate acyltransferase.腺苷酸激酶和sn-甘油-3-磷酸酰基转移酶缺陷型大肠杆菌突变体的遗传分析。
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Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.大肠杆菌K-12 W3110染色体有序黏粒文库的构建。
J Bacteriol. 1989 Feb;171(2):1214-8. doi: 10.1128/jb.171.2.1214-1218.1989.
J Bacteriol. 1980 Jan;141(1):405-8. doi: 10.1128/jb.141.1.405-408.1980.
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The conjugation system of F-like plasmids.F 类质粒的接合系统。
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[On a thermosensitive mutation of Escherichia coli affecting an energizing function].[关于大肠杆菌影响能量供应功能的一个温度敏感突变型]
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Chromosome transfer during bacterial mating.
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Selection and characterization of an E. coli mutant defective in membrane lipid biosynthesis.一种膜脂生物合成存在缺陷的大肠杆菌突变体的筛选与鉴定
Proc Natl Acad Sci U S A. 1970 Mar;65(3):737-44. doi: 10.1073/pnas.65.3.737.
8
Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.细菌细胞分裂调控:大肠杆菌dnaH基因座的特性分析
J Bacteriol. 1974 Aug;119(2):443-9. doi: 10.1128/jb.119.2.443-449.1974.
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Regulation of macromolecular biosynthesis in a mutant of Escherichia coli defective in membrane phospholipid biosynthesis.大肠杆菌膜磷脂生物合成缺陷突变体中大分子生物合成的调控。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):385-9. doi: 10.1073/pnas.70.2.385.
10
Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants.膜磷脂合成中具有温度敏感损伤的大肠杆菌突变体:甘油-3-磷酸酰基转移酶突变体的遗传分析
Mol Gen Genet. 1972;116(3):199-210. doi: 10.1007/BF00269765.