大肠杆菌K-12 W3110染色体有序黏粒文库的构建。
Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.
作者信息
Tabata S, Higashitani A, Takanami M, Akiyama K, Kohara Y, Nishimura Y, Nishimura A, Yasuda S, Hirota Y
机构信息
Department of Biology, Faculty of Science, Nagoya University, Japan.
出版信息
J Bacteriol. 1989 Feb;171(2):1214-8. doi: 10.1128/jb.171.2.1214-1218.1989.
Abstract
A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.
摘要
构建了大肠杆菌K-12 W3110染色体的黏粒文库，通过杂交和遗传标记互补试验将克隆定位到染色体图谱上的位置。该文库覆盖了约70%的基因组。所鉴定的克隆可在同源系统中保存，这将有助于大肠杆菌的遗传学研究。
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