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大肠杆菌K-12 W3110染色体有序黏粒文库的构建。

Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.

作者信息

Tabata S, Higashitani A, Takanami M, Akiyama K, Kohara Y, Nishimura Y, Nishimura A, Yasuda S, Hirota Y

机构信息

Department of Biology, Faculty of Science, Nagoya University, Japan.

出版信息

J Bacteriol. 1989 Feb;171(2):1214-8. doi: 10.1128/jb.171.2.1214-1218.1989.

DOI:10.1128/jb.171.2.1214-1218.1989
PMID:2644229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209726/
Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

摘要

构建了大肠杆菌K-12 W3110染色体的黏粒文库，通过杂交和遗传标记互补试验将克隆定位到染色体图谱上的位置。该文库覆盖了约70%的基因组。所鉴定的克隆可在同源系统中保存，这将有助于大肠杆菌的遗传学研究。

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本文引用的文献

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Toward a physical map of the genome of the nematode Caenorhabditis elegans.构建秀丽隐杆线虫基因组物理图谱。
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5. doi: 10.1073/pnas.83.20.7821.
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Utilization of d-Methionine by Escherichia coli.大肠杆菌对d-蛋氨酸的利用
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A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.一个控制大肠杆菌K-12中丝状形成和对辐射敏感性的基因座。
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Mutation that suppresses the protein export defect of the secY mutation and causes cold-sensitive growth of Escherichia coli.
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Gratuitous repression of avtA in Escherichia coli and Salmonella typhimurium.在大肠杆菌和鼠伤寒沙门氏菌中对avtA的无端抑制。
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Mapping of a locus (mdoA) that affects the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.对影响大肠杆菌中膜衍生寡糖生物合成的一个基因座（mdoA）的定位。
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Escherichia coli K-12 mutation that inactivates biodegradative threonine dehydratase by transposon Tn5 insertion.大肠杆菌K-12突变体，通过转座子Tn5插入使生物降解性苏氨酸脱水酶失活。
J Bacteriol. 1984 Jun;158(3):826-31. doi: 10.1128/jb.158.3.826-831.1984.
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Mol Gen Genet. 1981;184(2):208-12. doi: 10.1007/BF00272906.