Katz S M
Am J Pathol. 1978 Mar;90(3):701-22.
The Pennsylvania Department of Health, Bureau of Laboratories, has isolated the Legionnaires disease organism from two patients with Legionnaires' disease proved by serologic techniques. We have studied the morphology which the isolate assumes in yolk sac tissue and on bacteriologic media. The organism was Giménez-positive and gram-variable. Using an indirect immunofluorescent procedure, it was shown to react with convalescent serum samples taken from patients who had Legionnaires' disease. The organism multiplies by binary fission extracellularly and intracellulary; is both coccoid and bacillary in form; and contains characteristic cytoplasm, nucleoids, a cytoplasmic membrane, and a small cell wall of variable size. It may produce spores of unusual appearance. Intracellular replication characteristically occurs within vacuoles. The Legionnaires' disease organism conforms to the morphologic criteria for a prokaryocyte.
宾夕法尼亚州卫生部实验室局已从两名经血清学技术确诊为军团病的患者身上分离出军团病病原体。我们研究了该分离株在卵黄囊组织和细菌学培养基中的形态。该病原体吉姆萨染色呈阳性，革兰氏染色反应不定。采用间接免疫荧光法，结果显示它能与从患军团病患者采集的恢复期血清样本发生反应。该病原体通过二分裂在细胞外和细胞内繁殖；形态上既有球菌状又有杆菌状；含有特征性的细胞质、类核、细胞质膜和大小不一的小细胞壁。它可能产生外观异常的孢子。细胞内复制通常发生在液泡内。军团病病原体符合原核细胞的形态学标准。
