Glander H J, Dettmer D
Andrologia. 1978 Jan-Feb;10(1):69-73. doi: 10.1111/j.1439-0272.1978.tb01319.x.
A method for determination of the monosaccharide uptake into human spermatozoa is described. Intracellular monosaccharide concentrations were calculated on the basis of determination of intracellular radioactivity after incubation of the cells with labelled monosaccharide and using a mathematical procedure to approximate the intracellular space of spermatozoa. The D-fructose uptake depends on the extracellular D-fructose concentration in a hyperbolic manner. Half maximal saturation is present at 4,1 mM. This corresponds closely with the lowest limit of D-fructose concentration in human fertile semen.
本文描述了一种测定人类精子对单糖摄取的方法。在细胞与标记单糖孵育后，通过测定细胞内放射性，并使用数学方法估算精子的细胞内空间，计算细胞内单糖浓度。D-果糖的摄取以双曲线方式依赖于细胞外D-果糖浓度。半最大饱和度出现在4.1 mM处。这与人类可育精液中D-果糖浓度的最低限度密切相关。
