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通过两种方法从看似正常的猪扁桃体中分离出猪丹毒杆菌。

Isolation of Erysipelothrix rhusiopathiae from tonsils of apparently normal swine by two methods.

作者信息

Stephenson E H, Berman D T

出版信息

Am J Vet Res. 1978 Jan;39(1):187-8.

PMID:629445
Abstract

Erysipelothrix rhusiopathiae was isolated from the tonsils of 62 of 63 (98%) apparently normal pigs, using 2 procedures: (1) enrichment culture at 5 C followed by mouse inoculation, and (2) Wood's technique. There was no significant difference (P less than or equal to 0.01) in the sensitivity of erysipelothrix detection between the 2 procedures. Each of the isolates selected for identification had morphologic and tinctorial properties consistent with E rhusiopathiae and was identified by direct immunofluorescence.

摘要

采用两种方法从63头看似健康的猪中的62头(98%)的扁桃体中分离出猪红斑丹毒丝菌:(1)在5℃下进行增菌培养,随后接种小鼠;(2)采用伍德氏技术。两种方法在检测猪红斑丹毒丝菌的敏感性方面无显著差异(P≤0.01)。挑选用于鉴定的每一株分离菌都具有与猪红斑丹毒丝菌一致的形态和染色特性,并通过直接免疫荧光法进行鉴定。

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