牛乳头瘤病毒4型E8蛋白与连接蛋白结合，并导致原代成纤维细胞中缝隙连接的细胞间通讯丧失。
The bovine papillomavirus type 4 E8 protein binds to ductin and causes loss of gap junctional intercellular communication in primary fibroblasts.
作者信息
Faccini A M, Cairney M, Ashrafi G H, Finbow M E, Campo M S, Pitts J D
机构信息
Beatson Laboratories, Beatson Institute for Cancer Research, Bearsden, Glasgow, United Kingdom.
出版信息
J Virol. 1996 Dec;70(12):9041-5. doi: 10.1128/JVI.70.12.9041-9045.1996.
Abstract
The E8 open reading frame of bovine papillomavirus type 4 encodes a small hydrophobic polypeptide which contributes to cell transformation by conferring anchorage-independent growth. Using an in vitro translation system, we show that the E8 polypeptide binds to ductin, the 16-kDa proteolipid that forms transmembrane channels in both gap junctions and vacuolar H+-ATPase. This association is not due to nonspecific hydrophobic interactions. PPA1, a Saccharomyces cerevisiae polypeptide homologous (with 25% identity) to ductin, does not complex with E8. Furthermore, E5B, structurally similar to E8 but with no transforming activity, does not form a complex with ductin. Primary bovine fibroblasts expressing E8 show a loss of gap junctional intercellular communication, and it is suggested that this results from the interaction between E8 and ductin.
摘要
牛乳头瘤病毒4型的E8开放阅读框编码一种小的疏水性多肽，该多肽通过赋予不依赖贴壁生长的特性来促进细胞转化。利用体外翻译系统，我们发现E8多肽与导管蛋白结合，导管蛋白是一种16 kDa的蛋白脂质，在间隙连接和液泡H⁺-ATP酶中均形成跨膜通道。这种结合并非由于非特异性疏水相互作用。PPA1是一种与导管蛋白同源（具有25%的同一性）的酿酒酵母多肽，它不与E8形成复合物。此外，E5B在结构上与E8相似，但没有转化活性，它也不与导管蛋白形成复合物。表达E8的原代牛成纤维细胞显示间隙连接细胞间通讯丧失，提示这是E8与导管蛋白相互作用的结果。
相似文献
1
The bovine papillomavirus type 4 E8 protein binds to ductin and causes loss of gap junctional intercellular communication in primary fibroblasts.牛乳头瘤病毒4型E8蛋白与连接蛋白结合，并导致原代成纤维细胞中缝隙连接的细胞间通讯丧失。
J Virol. 1996 Dec;70(12):9041-5. doi: 10.1128/JVI.70.12.9041-9045.1996.
2
Binding of bovine papillomavirus type 4 E8 to ductin (16K proteolipid), down-regulation of gap junction intercellular communication and full cell transformation are independent events.
J Gen Virol. 2000 Mar;81(Pt 3):689-94. doi: 10.1099/0022-1317-81-3-689.
3
A mutational analysis of the transforming functions of the E8 protein of bovine papillomavirus type 4.
Virology. 1999 Mar 15;255(2):385-94. doi: 10.1006/viro.1998.9563.
4
Induction of cell transformation by mutated 16K vacuolar H+-atpase (ductin) is accompanied by down-regulation of gap junctional intercellular communication and translocation of connexin 43 in NIH3T3 cells.突变型16K液泡H⁺-ATP酶（导管蛋白）诱导细胞转化伴随着NIH3T3细胞中缝隙连接细胞间通讯的下调和连接蛋白43的易位。
Oncogene. 1998 Oct 1;17(13):1673-80. doi: 10.1038/sj.onc.1202092.
5
Evidence that the 16 kDa proteolipid (subunit c) of the vacuolar H(+)-ATPase and ductin from gap junctions are the same polypeptide in Drosophila and Manduca: molecular cloning of the Vha16k gene from Drosophila.液泡H(+) -ATP酶的16 kDa蛋白脂质（亚基c）与果蝇和烟草天蛾中缝隙连接的导管蛋白是同一多肽的证据：果蝇Vha16k基因的分子克隆
J Cell Sci. 1994 Jul;107 ( Pt 7):1817-24. doi: 10.1242/jcs.107.7.1817.
6
Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis.质子泵与间隙连接的关联：在果蝇卵子发生过程中，V-ATP酶的通道形成亚基c（导管蛋白）与亚基a以及与连接蛋白2和3的共定位。
BMC Dev Biol. 2016 Jul 13;16(1):24. doi: 10.1186/s12861-016-0124-y.
7
Ductin--a proton pump component, a gap junction channel and a neurotransmitter release channel.导管蛋白——一种质子泵成分、一种间隙连接通道和一种神经递质释放通道。
Bioessays. 1995 Mar;17(3):247-55. doi: 10.1002/bies.950170311.
8
Disposition and orientation of ductin (DCCD-reactive vacuolar H(+)-ATPase subunit) in mammalian membrane complexes.导管蛋白（二环己基碳二亚胺反应性液泡H(+) -ATP酶亚基）在哺乳动物膜复合物中的分布与取向
Exp Cell Res. 1993 Aug;207(2):261-70. doi: 10.1006/excr.1993.1192.
9
Is the gap junction channel--the connexon--made of connexin or ductin?间隙连接通道——连接子——是由连接蛋白还是导管蛋白构成的？
J Cell Sci. 1993 Oct;106 ( Pt 2):463-71. doi: 10.1242/jcs.106.2.463.
10
Membrane insertion and assembly of ductin: a polytopic channel with dual orientations.导管蛋白的膜插入与组装：一种具有双重取向的多跨膜通道。
EMBO J. 1995 Aug 1;14(15):3609-16. doi: 10.1002/j.1460-2075.1995.tb00030.x.
引用本文的文献
1
Mechanisms of Intracellular Communication in Cancer and Pathogen Spreading.癌细胞内通讯和病原体传播的机制。
Results Probl Cell Differ. 2024;73:301-326. doi: 10.1007/978-3-031-62036-2_13.
2
Acute Adenoviral Infection Elicits an Arrhythmogenic Substrate Prior to Myocarditis.急性腺病毒感染在心肌炎发生之前引发致心律失常基质。
Circ Res. 2024 Mar 29;134(7):892-912. doi: 10.1161/CIRCRESAHA.122.322437. Epub 2024 Feb 28.
3
. and Bovine Papillomavirus: Partners in Cancer.以及牛乳头瘤病毒：癌症中的伙伴。
Front Vet Sci. 2021 Nov 2;8:758720. doi: 10.3389/fvets.2021.758720. eCollection 2021.
4
Connexin 43/47 channels are important for astrocyte/ oligodendrocyte cross-talk in myelination and demyelination.连接蛋白43/47通道对于髓鞘形成和脱髓鞘过程中星形胶质细胞/少突胶质细胞的相互作用至关重要。
J Biosci. 2018 Dec;43(5):1055-1068. doi: 10.1007/s12038-018-9811-0.
5
Loss of Cx43-Mediated Functional Gap Junction Communication in Meningeal Fibroblasts Following Mouse Hepatitis Virus Infection.在小鼠肝炎病毒感染后，脑膜成纤维细胞中 Cx43 介导的功能性缝隙连接通讯丧失。
Mol Neurobiol. 2018 Aug;55(8):6558-6571. doi: 10.1007/s12035-017-0861-3. Epub 2018 Jan 11.
6
Mouse Hepatitis Virus Infection Remodels Connexin43-Mediated Gap Junction Intercellular Communication In Vitro and In Vivo.小鼠肝炎病毒感染在体外和体内重塑了连接蛋白43介导的间隙连接细胞间通讯。
J Virol. 2015 Dec 16;90(5):2586-99. doi: 10.1128/JVI.02420-15.
7
Effect of human immunodeficiency virus on blood-brain barrier integrity and function: an update.人类免疫缺陷病毒对血脑屏障完整性和功能的影响：最新进展
Front Cell Neurosci. 2015 Jun 10;9:212. doi: 10.3389/fncel.2015.00212. eCollection 2015.
8
Regulation of gap junction channels by infectious agents and inflammation in the CNS.中枢神经系统中感染因子和炎症对缝隙连接通道的调节。
Front Cell Neurosci. 2014 May 9;8:122. doi: 10.3389/fncel.2014.00122. eCollection 2014.
9
Role of connexin/pannexin containing channels in infectious diseases.连接蛋白/间隙连接蛋白通道在传染病中的作用。
FEBS Lett. 2014 Apr 17;588(8):1389-95. doi: 10.1016/j.febslet.2014.01.030. Epub 2014 Jan 28.
10
The E5 proteins.E5 蛋白。
Virology. 2013 Oct;445(1-2):99-114. doi: 10.1016/j.virol.2013.05.006. Epub 2013 May 31.
本文引用的文献
1
Cell-cell communication and growth control of normal and cancer cells: evidence and hypothesis.正常细胞与癌细胞的细胞间通讯及生长控制：证据与假说
Mol Carcinog. 1993;7(1):14-7. doi: 10.1002/mc.2940070103.
2
The E5 oncoprotein of human papillomavirus type 16 transforms fibroblasts and effects the downregulation of the epidermal growth factor receptor in keratinocytes.人乳头瘤病毒16型的E5癌蛋白可转化成纤维细胞，并影响角质形成细胞中表皮生长因子受体的下调。
J Virol. 1993 Aug;67(8):4521-32. doi: 10.1128/JVI.67.8.4521-4532.1993.
3
Genetic definition of a new bovine papillomavirus type 1 open reading frame, E5B, that encodes a hydrophobic protein involved in altering host-cell protein processing.一种新的牛乳头瘤病毒1型开放阅读框E5B的遗传学定义，该开放阅读框编码一种参与改变宿主细胞蛋白质加工的疏水蛋白。
J Virol. 1993 Jun;67(6):3427-34. doi: 10.1128/JVI.67.6.3427-3434.1993.
4
Analysis of the transforming functions of bovine papillomavirus type 4.牛乳头瘤病毒4型转化功能的分析
Virology. 1993 Apr;193(2):614-20. doi: 10.1006/viro.1993.1169.
5
Disposition and orientation of ductin (DCCD-reactive vacuolar H(+)-ATPase subunit) in mammalian membrane complexes.导管蛋白（二环己基碳二亚胺反应性液泡H(+) -ATP酶亚基）在哺乳动物膜复合物中的分布与取向
Exp Cell Res. 1993 Aug;207(2):261-70. doi: 10.1006/excr.1993.1192.
6
Is the gap junction channel--the connexon--made of connexin or ductin?间隙连接通道——连接子——是由连接蛋白还是导管蛋白构成的？
J Cell Sci. 1993 Oct;106 ( Pt 2):463-71. doi: 10.1242/jcs.106.2.463.
7
The human T-cell leukemia/lymphotropic virus type I p12I protein cooperates with the E5 oncoprotein of bovine papillomavirus in cell transformation and binds the 16-kilodalton subunit of the vacuolar H+ ATPase.人类I型嗜T细胞白血病/淋巴瘤病毒p12I蛋白在细胞转化过程中与牛乳头瘤病毒E5癌蛋白协同作用，并与液泡H⁺ATP酶的16千道尔顿亚基结合。
J Virol. 1993 Dec;67(12):7701-4. doi: 10.1128/JVI.67.12.7701-7704.1993.
8
Experimental reproduction of the papilloma-carcinoma complex of the alimentary canal in cattle.牛消化道乳头瘤-癌复合体的实验性复制
Carcinogenesis. 1994 Aug;15(8):1597-601. doi: 10.1093/carcin/15.8.1597.
9
Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line.人乳头瘤病毒16型E5蛋白影响上皮细胞系中的细胞间通讯。
J Virol. 1995 Jul;69(7):4489-94. doi: 10.1128/JVI.69.7.4489-4494.1995.
10
The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes.人乳头瘤病毒16型的E5癌蛋白可抑制人角质形成细胞中内体的酸化。
J Virol. 1995 May;69(5):3185-92. doi: 10.1128/JVI.69.5.3185-3192.1995.
Zotero 插件