人类肺泡巨噬细胞产生白三烯B4。
Human alveolar macrophages produce leukotriene B4.
作者信息
Fels A O, Pawlowski N A, Cramer E B, King T K, Cohn Z A, Scott W A
出版信息
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7866-70. doi: 10.1073/pnas.79.24.7866.
Abstract
Human alveolar macrophages obtained by bronchoalveolar lavage were labeled overnight with [3H]arachidonic acid. The cells were stimulated with calcium ionophore A23187, and the 20:4 oxygenated metabolites released into the culture medium were identified by reverse-phase HPLC. Leukotriene B4 was the major 20:4 metabolite produced by these cultures. Leukotriene B4 was identified by its reverse-phase HPLC elution time, its UV spectrum, and its chemotactic and chemokinetic activities for neutrophils. In addition, the macrophage- and neutrophil-derived leukotriene B4 free acids and methyl esters were found to have identical HPLC retention times.
摘要
通过支气管肺泡灌洗获得的人肺泡巨噬细胞用[3H]花生四烯酸标记过夜。用钙离子载体A23187刺激细胞，通过反相高效液相色谱法鉴定释放到培养基中的20:4氧化代谢产物。白三烯B4是这些培养物产生的主要20:4代谢产物。通过其反相高效液相色谱洗脱时间、紫外光谱以及对中性粒细胞的趋化和化学动力学活性来鉴定白三烯B4。此外，发现巨噬细胞和中性粒细胞衍生的白三烯B4游离酸和甲酯具有相同的高效液相色谱保留时间。
相似文献
1
Human alveolar macrophages produce leukotriene B4.人类肺泡巨噬细胞产生白三烯B4。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7866-70. doi: 10.1073/pnas.79.24.7866.
2
Relative contribution of leukotriene B4 to the neutrophil chemotactic activity produced by the resident human alveolar macrophage.白三烯B4对人肺泡巨噬细胞产生的中性粒细胞趋化活性的相对贡献。
J Clin Invest. 1987 Oct;80(4):1114-24. doi: 10.1172/JCI113168.
3
Arachidonic acid metabolism in bovine alveolar macrophages. Effect of calcium ionophore on lipoxygenase products.牛肺泡巨噬细胞中的花生四烯酸代谢。钙离子载体对脂氧合酶产物的影响。
Inflammation. 1986 Jun;10(2):157-65. doi: 10.1007/BF00915997.
4
5
Leukotriene B4 is an incomplete agonist for the activation of human neutrophils.白三烯B4是激活人类中性粒细胞的不完全激动剂。
Biochem Biophys Res Commun. 1984 Jul 31;122(2):535-41. doi: 10.1016/s0006-291x(84)80066-2.
6
Stimulation of arachidonic acid metabolism by adherence of alveolar macrophages to a plastic substrate. Modulation by fetal bovine serum.肺泡巨噬细胞黏附于塑料基质对花生四烯酸代谢的刺激作用。胎牛血清的调节作用。
Am Rev Respir Dis. 1988 Jan;137(1):38-43. doi: 10.1164/ajrccm/137.1.38.
7
Guinea pig alveolar eosinophils and macrophages produce leukotriene B4 but no peptido-leukotriene.豚鼠肺泡嗜酸性粒细胞和巨噬细胞产生白三烯B4，但不产生肽白三烯。
J Immunol. 1990 Mar 1;144(5):1880-5.
8
Characterization of arachidonic acid metabolism, superoxide production, and bacterial killing by bovine alveolar neutrophils elicited with leukotriene B4 and zymosan-activated plasma.白三烯B4和酵母聚糖激活血浆诱导的牛肺泡中性粒细胞的花生四烯酸代谢、超氧化物生成及细菌杀伤特性
Inflammation. 1991 Feb;15(1):31-42. doi: 10.1007/BF00917907.
9
Rat alveolar macrophages synthesize leukotriene B4 and 12-hydroxyeicosatetraenoic acid from alveolar epithelial cell-derived arachidonic acid.
Am Rev Respir Dis. 1988 Oct;138(4):928-35. doi: 10.1164/ajrccm/138.4.928.
10
Production of arachidonic acid metabolites by caprine alveolar macrophages.山羊肺泡巨噬细胞花生四烯酸代谢产物的产生
Inflammation. 1990 Jun;14(3):239-45. doi: 10.1007/BF00915808.
引用本文的文献
1
Microphysiological Systems for Studying Cellular Crosstalk During the Neutrophil Response to Infection.用于研究中性粒细胞对感染反应过程中细胞串扰的微生理系统。
Front Immunol. 2021 Apr 27;12:661537. doi: 10.3389/fimmu.2021.661537. eCollection 2021.
2
Leukotrienes in pulmonary arterial hypertension.肺动脉高压中的白三烯
Immunol Res. 2014 May;58(2-3):387-93. doi: 10.1007/s12026-014-8492-5.
3
Bifunctional lipocalin ameliorates murine immune complex-induced acute lung injury.双功能载脂蛋白减轻小鼠免疫复合物诱导的急性肺损伤。
J Biol Chem. 2013 Jun 28;288(26):18789-802. doi: 10.1074/jbc.M112.420331. Epub 2013 Apr 26.
4
Leukotriene B4, administered via intracerebroventricular injection, attenuates the antigen-induced asthmatic response in sensitized guinea pigs.白三烯 B4 通过脑室内注射给药，可减轻致敏豚鼠的抗原诱导的哮喘反应。
J Neuroinflammation. 2010 Feb 11;7:12. doi: 10.1186/1742-2094-7-12.
5
Enhanced levels of leukotriene B(4) in synovial fluid in Lyme disease.莱姆病患者滑液中白三烯 B4 水平升高。
Mediators Inflamm. 1993;2(3):225-8. doi: 10.1155/S0962935193000304.
6
Leukotriene inhibition in hamster periodontitis. A histochemical and morphometric study.白三烯抑制在仓鼠牙周炎中的作用。组织化学和形态计量学研究。
Mediators Inflamm. 1992;1(5):335-9. doi: 10.1155/S0962935192000504.
7
Determinants of the cellular specificity of acetaminophen as an inhibitor of prostaglandin H(2) synthases.对乙酰氨基酚作为前列腺素H(2)合成酶抑制剂的细胞特异性的决定因素。
Proc Natl Acad Sci U S A. 2002 May 14;99(10):7130-5. doi: 10.1073/pnas.102588199.
8
The coordinated action of CC chemokines in the lung orchestrates allergic inflammation and airway hyperresponsiveness.CC趋化因子在肺部的协同作用调控着过敏性炎症和气道高反应性。
J Exp Med. 1998 Jul 6;188(1):157-67. doi: 10.1084/jem.188.1.157.
9
Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines, and adhesion receptors.嗜酸性粒细胞在小鼠过敏性炎症模型中向肺部的募集。T细胞、趋化因子和黏附受体的作用。
J Clin Invest. 1996 Nov 15;98(10):2332-45. doi: 10.1172/JCI119045.
10
Inhibition of leukotriene B4-receptor interaction suppresses eosinophil infiltration and disease pathology in a murine model of experimental allergic encephalomyelitis.抑制白三烯B4受体相互作用可抑制实验性变应性脑脊髓炎小鼠模型中的嗜酸性粒细胞浸润和疾病病理。
J Exp Med. 1996 Apr 1;183(4):1893-8. doi: 10.1084/jem.183.4.1893.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Alveolar macrophage-derived chemotactic factor: kinetics of in vitro production and partial characterization.肺泡巨噬细胞衍生趋化因子：体外产生的动力学及部分特性
J Clin Invest. 1980 Feb;65(2):268-76. doi: 10.1172/JCI109668.
3
Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly.中性粒细胞趋化性的快速定量：在多孔组件中使用无聚乙烯吡咯烷酮的聚碳酸酯膜。
J Immunol Methods. 1980;37(1):39-45. doi: 10.1016/0022-1759(80)90179-9.
4
A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration.一种用于快速准确测量白细胞迁移的48孔微量化学趋化性组件。
J Immunol Methods. 1980;33(3):239-47. doi: 10.1016/0022-1759(80)90211-2.
5
Uptake and metabolism of monohydroxy-eicosatetraenoic acids by macrophages.巨噬细胞对单羟基二十碳四烯酸的摄取与代谢
J Exp Med. 1982 Jun 1;155(6):1653-64. doi: 10.1084/jem.155.6.1653.
6
Mouse peritoneal macrophages release leukotriene C in response to a phagocytic stimulus.小鼠腹腔巨噬细胞在吞噬刺激下释放白三烯C。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4928-32. doi: 10.1073/pnas.77.8.4928.
7
Prostaglandin synthesis by macrophages requires a specific receptor-ligand interaction.巨噬细胞合成前列腺素需要特定的受体-配体相互作用。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4279-82. doi: 10.1073/pnas.77.7.4279.
8
Resting macrophages produce distinct metabolites from exogenous arachidonic acid.静息巨噬细胞从外源性花生四烯酸产生独特的代谢产物。
J Exp Med. 1982 Feb 1;155(2):535-47. doi: 10.1084/jem.155.2.535.
9
Identification and biological activity of novel omega-oxidized metabolites of leukotriene B4 from human leukocytes.人白细胞中白三烯B4新型ω-氧化代谢产物的鉴定及其生物学活性
FEBS Lett. 1981 Jul 20;130(1):107-12. doi: 10.1016/0014-5793(81)80676-x.
10
Secretion of leukotriene C and other arachidonic acid metabolites by macrophages challenged with immunoglobulin E immune complexes.用免疫球蛋白E免疫复合物刺激巨噬细胞后，其白三烯C及其他花生四烯酸代谢产物的分泌情况。
J Exp Med. 1982 Oct 1;156(4):1077-86. doi: 10.1084/jem.156.4.1077.
Zotero 插件