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念珠藻PCC7524，一种含有五种序列特异性脱氧核糖核酸酶的蓝细菌。

Nostoc PCC7524, a cyanobacterium which contains five sequence-specific deoxyribonucleases.

作者信息

Reaston J, Duyvesteyn G C, de Waard A

出版信息

Gene. 1982 Nov;20(1):103-10. doi: 10.1016/0378-1119(82)90091-9.

DOI:10.1016/0378-1119(82)90091-9
PMID:6298063
Abstract

Five nucleotide sequence-specific deoxyribonucleases present in cell-free extracts of the filamentous cyanobacterium Nostoc PCC7524 have been purified and characterized. One of these enzymes, designated Nsp(7524)I cleaves at a new kind of nucleotide sequence, i.e. 5'-PuCATG Py-3'. The other four restriction enzymes in this organism, designated Nsp(7524)II, Nsp(7524)III, Nsp(7524)IV and Nsp(7524)V, are isoschizomers of enzymes which have been previously described. The cleavage site of Nsp(7524)II which is an isoschizomer of SduI was determined.

摘要

丝状蓝细菌集胞藻PCC7524无细胞提取物中存在的5种核苷酸序列特异性脱氧核糖核酸酶已被纯化并进行了表征。其中一种酶，命名为Nsp(7524)I，能在一种新的核苷酸序列处切割，即5'-PuCATG Py-3'。该生物体中的其他四种限制酶，命名为Nsp(7524)II、Nsp(7524)III、Nsp(7524)IV和Nsp(7524)V，是先前已描述的酶的同裂酶。确定了作为SduI同裂酶的Nsp(7524)II的切割位点。

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引用本文的文献

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