一种从集胞藻6701中识别脱氧核苷酸序列CCNNGG的新型核酸内切酶。
A new endonuclease recognizing the deoxynucleotide sequence CCNNGG from the cyanobacterium Synechocystis 6701.
作者信息
Calléja F, Tandeau de Marsac N, Coursin T, van Ormondt H, de Waard A
出版信息
Nucleic Acids Res. 1985 Sep 25;13(18):6745-51. doi: 10.1093/nar/13.18.6745.
Abstract
A new sequence-specific endonuclease from the cyanobacterium Synechocystis species PCC 6701 has been purified and characterized. This enzyme, SecI, is unique in recognizing the nucleotide sequence: 5' -CCNNGG-3' 3' -GGNNCC-5' and cleaves it at the position indicated by the symbol. Two other restriction endonucleases, SecII and SecIII, found in this organism are isoschizomers of MspI and MstII, respectively.
摘要
一种来自蓝藻集胞藻PCC 6701的新型序列特异性内切核酸酶已被纯化并进行了表征。这种酶，即SecI，在识别核苷酸序列5'-CCNNGG-3' 3'-GGNNCC-5'方面具有独特性，并在符号所示位置进行切割。在该生物体中发现的另外两种限制性内切核酸酶SecII和SecIII分别是MspI和MstII的同裂酶。
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