通过Northern印迹杂交对轮状病毒双链RNA片段进行基因定位：应用于第7、8和9片段
Gene mapping of rotavirus double-stranded RNA segments by northern blot hybridization: application to segments 7, 8, and 9.
作者信息
Dyall-Smith M L, Azad A A, Holmes I H
出版信息
J Virol. 1983 Apr;46(1):317-20. doi: 10.1128/JVI.46.1.317-320.1983.
Abstract
Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [alpha-(32)P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.
摘要
用[α-(32)P]ATP对英国牛轮状病毒双链RNA片段7、8和9的克隆DNA拷贝进行切口平移，然后与在长聚丙烯酰胺凝胶上分离后转移到邻氨基苯硫醚纸上的各种轮状病毒株的双链RNA杂交。英国小牛克隆与分离的RNA片段的特异性杂交使得能够快速确定四种不同轮状病毒的相应基因。
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