非洲爪蟾成年次要珠蛋白多肽编码mRNA的分子克隆与测序
Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.
作者信息
Knöchel W, Meyerhof W, Hummel S, Grundmann U
出版信息
Nucleic Acids Res. 1983 Mar 11;11(5):1543-53. doi: 10.1093/nar/11.5.1543.
Abstract
Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult alpha and beta globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.
摘要
从成年非洲爪蟾雌性未成熟红细胞中分离出珠蛋白信使核糖核酸（Globin mRNA）。信使核糖核酸/互补脱氧核糖核酸（mRNA/cDNA）杂交体通过G/C加尾整合到pBR 322的Pst I切割位点，并克隆到大肠杆菌HB 101菌株中。通过限制性酶切位点分析以及杂交行为，我们鉴定出两个编码次要成年α和β珠蛋白链的克隆。本文给出了核苷酸序列分析及推导的氨基酸序列。
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本文引用的文献
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