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非洲爪蟾成年次要珠蛋白多肽编码mRNA的分子克隆与测序

Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis.

作者信息

Knöchel W, Meyerhof W, Hummel S, Grundmann U

出版信息

Nucleic Acids Res. 1983 Mar 11;11(5):1543-53. doi: 10.1093/nar/11.5.1543.

DOI:10.1093/nar/11.5.1543
PMID:6298748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325814/
Abstract

Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult alpha and beta globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.

摘要

从成年非洲爪蟾雌性未成熟红细胞中分离出珠蛋白信使核糖核酸（Globin mRNA）。信使核糖核酸/互补脱氧核糖核酸（mRNA/cDNA）杂交体通过G/C加尾整合到pBR 322的Pst I切割位点，并克隆到大肠杆菌HB 101菌株中。通过限制性酶切位点分析以及杂交行为，我们鉴定出两个编码次要成年α和β珠蛋白链的克隆。本文给出了核苷酸序列分析及推导的氨基酸序列。

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引用本文的文献

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Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.根据cDNA序列预测的来自非洲爪蟾表皮的分子量为51000的酸性I型细胞角蛋白羧基末端部分的氨基酸序列。
EMBO J. 1984 Jun;3(6):1301-6. doi: 10.1002/j.1460-2075.1984.tb01966.x.
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The pattern of expression of the Xenopus laevis tadpole alpha-globin genes and the amino acid sequence of the three major tadpole alpha-globin polypeptides.非洲爪蟾蝌蚪α-珠蛋白基因的表达模式以及三种主要蝌蚪α-珠蛋白多肽的氨基酸序列。
Nucleic Acids Res. 1985 Aug 12;13(15):5407-21. doi: 10.1093/nar/13.15.5407.

本文引用的文献

1
The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.非洲爪蟾主要β-珠蛋白信使核糖核酸的核苷酸序列。
Nucleic Acids Res. 1980 Sep 25;8(18):4247-58. doi: 10.1093/nar/8.18.4247.
2
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.非洲爪蟾中成年α-珠蛋白基因和β-珠蛋白基因的连锁以及四倍体化导致的基因复制。
Cell. 1980 Sep;21(2):555-64. doi: 10.1016/0092-8674(80)90493-6.
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[Hemoglobins, XLII: Studies on the hemoglobin of the greylag goose (Anser anser). The primary structures of the alpha- and beta-chains of the main component (author's transl)].[血红蛋白，XLII：灰雁（Anser anser）血红蛋白的研究。主要成分α链和β链的一级结构（作者译）]
Hoppe Seylers Z Physiol Chem. 1981 Aug;362(8):1101-12.
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No evidence for 'stress' alpha-globin genes in chicken.鸡中不存在“应激”α-珠蛋白基因的证据。
Nature. 1982 Feb 25;295(5851):710-2. doi: 10.1038/295710a0.
5
Molecular cloning of cDNA sequences coding for the major alpha- and beta-globin polypeptides of adult Xenopus laevis.成年非洲爪蟾主要α和β珠蛋白多肽编码cDNA序列的分子克隆
Nucleic Acids Res. 1980 Jun 25;8(12):2691-707. doi: 10.1093/nar/8.12.2691.
6
The Xenopus laevis globin gene family: chromosomal arrangement and gene structure.非洲爪蟾珠蛋白基因家族：染色体排列与基因结构
Cell. 1983 Jan;32(1):45-53. doi: 10.1016/0092-8674(83)90495-6.
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Complete nucleotide sequence of a cloned cDNA derived from the major adult alpha-globin mRNA of X. laevis.源自非洲爪蟾主要成年α-珠蛋白mRNA的克隆cDNA的完整核苷酸序列。
Nucleic Acids Res. 1983 Mar 11;11(5):1537-42. doi: 10.1093/nar/11.5.1537.
8
Partial sequence analysis of Xenopus alpha- and beta-globin mRNA as determined from recombinant DNA plasmids.从重组DNA质粒中测定非洲爪蟾α-和β-珠蛋白mRNA的部分序列分析。
Dev Biol. 1980 Jul;78(1):161-72. doi: 10.1016/0012-1606(80)90326-7.
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Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
10
Comparative analysis of cloned larval and adult globin cDNA sequences of Xenopus laevis.非洲爪蟾克隆的幼虫和成体珠蛋白cDNA序列的比较分析。
Dev Biol. 1981 Dec;88(2):325-32. doi: 10.1016/0012-1606(81)90176-7.