猫科动物细胞系中肠道冠状病毒的研究。
Studies of enteric coronaviruses in a feline cell line.
作者信息
Woods R D
出版信息
Vet Microbiol. 1982 Nov;7(5):427-35. doi: 10.1016/0378-1135(82)90059-1.
Abstract
Development is reported of a feline cell line which can support the growth of coronaviruses from canine (CCV), feline (FIPV) and porcine (TGEV) species. The cell culture has been serially transferred over 100 times and has retained its initial growth requirements, proliferative capacity and morphologic features. Each virus had specific growth characteristics in this cell culture although all produced a similar CPE and plaques under agar. Cross neutralization studies demonstrated a two-way relationship between TGEV and CCV and between TGEV and FIPV, whereas a one-way relationship was demonstrated between CCV and FIPV.
摘要
据报道，已培育出一种猫细胞系，它能够支持犬冠状病毒（CCV）、猫冠状病毒（FIPV）和猪传染性胃肠炎病毒（TGEV）的生长。该细胞培养物已连续传代100多次，仍保持其最初的生长需求、增殖能力和形态特征。每种病毒在这种细胞培养物中都有特定的生长特性，尽管在琼脂平板上所有病毒都产生了相似的细胞病变效应（CPE）和蚀斑。交叉中和试验表明，TGEV与CCV之间以及TGEV与FIPV之间存在双向关系，而CCV与FIPV之间存在单向关系。
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