Kahn H J, Marks A, Thom H, Baumal R
Am J Clin Pathol. 1983 Mar;79(3):341-7. doi: 10.1093/ajcp/79.3.341.
Normal tissues and various tumors were examined for S100 protein, using anti-S100 protein antiserum, in an immunoperoxidase reaction. Among normal tissues, in addition to the previously reported presence of S100 protein in some neurons, glial, and Schwann cells of the nervous system, melanocytes and Langerhans cells of the skin, interdigitating reticulum cells of lymph nodes, and chondrocytes, we demonstrated it in myoepithelial cells and ducts of sweat glands, salivary glands, and the breast, serous glands of the lung, fetal neuroblasts, and sustentacular cells of the adrenal medulla. Among neoplasms, S100 protein previously has been reported in neurogenic tumors, melanomas, and neuroblastomas; we have demonstrated it in mixed sweat gland tumors, histiocytosis X, pleomorphic adenomas of the salivary gland, medullary carcinomas of the breast, bronchioloalveolar carcinomas of the lung, sustentacular cells of pheochromocytomas, teratomas of the ovary, and tumors of cartilage (enchondromas, osteochondromas, and chondrosarcomas). With S100 protein producing tumors, a normal progenitor cell was identified, indicating that demonstration of S100 protein in tumors confirmed their origin.
使用抗S100蛋白抗血清，通过免疫过氧化物酶反应检测正常组织和各种肿瘤中的S100蛋白。在正常组织中，除了先前报道的神经系统的一些神经元、神经胶质细胞和施万细胞、皮肤的黑素细胞和朗格汉斯细胞、淋巴结的指状网状细胞以及软骨细胞中存在S100蛋白外，我们还在肌上皮细胞以及汗腺、唾液腺和乳腺的导管、肺的浆液腺、胎儿成神经细胞和肾上腺髓质的支持细胞中证实了其存在。在肿瘤中，先前已报道神经源性肿瘤、黑色素瘤和成神经细胞瘤中存在S100蛋白；我们在混合性汗腺肿瘤、组织细胞增多症X、唾液腺多形性腺瘤、乳腺髓样癌、肺细支气管肺泡癌、嗜铬细胞瘤的支持细胞、卵巢畸胎瘤和软骨肿瘤（内生软骨瘤、骨软骨瘤和软骨肉瘤）中也证实了其存在。对于产生S100蛋白的肿瘤，鉴定出了正常祖细胞，这表明在肿瘤中检测到S100蛋白证实了它们的起源。
