克隆于大肠杆菌中的乙肝病毒DNA的限制酶切图谱。
Restriction map of the hepatitis B virus DNA cloned in Escherichia coli.
作者信息
Bichko V V, Kozlovskaya T M, Dishler A, Pumpen P, Janulaitis A, Gren E J
出版信息
Gene. 1982 Dec;20(3):481-4. doi: 10.1016/0378-1119(82)90218-9.
PMID:6299904
Abstract
A plasmid carrying the complete genome of hepatitis B virus (HBV) inserted into the BamHI site of the pBR322 plasmid vector has been constructed. The physical map of HBV DNA established for 13 restriction endonucleases allows to conclude that the cloned DNA is similar, but not identical to the HBV DNA of ayw subtype.
摘要
已构建了一种质粒，它将乙型肝炎病毒（HBV）的完整基因组插入到pBR322质粒载体的BamHI位点。针对13种限制性内切酶建立的HBV DNA物理图谱表明，克隆的DNA与ayw亚型的HBV DNA相似，但并不相同。
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